染料木素对去卵巢大鼠颌骨代谢的改善作用

The beneficial effect of genistein on mandible bone metabolism in ovariectomized rats

目的 探讨染料木素对去势大鼠颌骨代谢及雌性生殖系统的作用。方法 切除成年雌性大鼠双侧卵巢 ,造成绝经后骨质疏松的动物模型。双能X线法检测颌骨、股骨骨密度 ,生化法检测血清碱性磷酸酶 (alkalinephosphatase ,ALP)、酸性磷酸酶 (acidphosphatase ,ACP) ,放射免疫法检测骨代谢因子IL 1β、IL 6、转化生长因子α(tumornecrosisfactor α ,TNF α)、降钙素和骨钙素 ,探讨染料木素对去势大鼠颌骨代谢的作用 ;通过血清雌二醇含量和子宫重量的变化监测染料木素的雌激素样副作用。结果 去势大鼠骨密度显著减少 ;血清ALP、ACP和IL 1β、骨钙素显著高于非去势组。口服染料木素后 ,大鼠骨密度显著增加 ;血清ALP、ACP、骨钙素显著增高 ,其中ALP和骨钙素的增加显著优于雌激素组 ,IL 1β和TNF α显著降低 ;大鼠子宫重量系数显著低于雌激素组。 结论 染料木素能够通过抑制骨吸收和促进骨形成有效改善去势大鼠颌骨代谢 ,副作用弱于雌激素。

Objective To detect the effect of genistein on mandible metabolism in ovariectomized rats. Methods Forty 12 week-old female SD rats were randomly assigned to four groups: ①sham operated; ②ovariectomized; ③ovariectomized and treated with estradiol; ④ovariectomized and received genistein, 45 mg/kg body weight per day. After 12 weeks, bone mineral density (BMD), serum level of alkaline phosphatase (ALP), acid phosphatase (ACP), osteocalcin, IL-1β, TNF-α,IL-6 and calcitonin (CT) were evaluated. In addition, the serum estradiol and the weight of uteri were also examined to indicate the side effect of genistein to the uteri. Results Ovariectomized animals had a significant decrease in BMD, and increased serum level of ALP, ACP, IL-1β and osteocalcin compared with sham rats. After treated with genistein, BMD and the serum level of ALP, ACP, osteocalcin increased significantly, while the serum level of IL-1β and TNF-α decreased. Especially, the increase of ALP and osteocalcin was higher than that of estradiol-treated animal. Additionally, the uterus weight index and the serum estradiol in genistein-treated rats were lower significantly than those of estradiol-treated rats. Conclusions Genistein can improve the mandible bone metabolism as well as its effect on femur through the promotion of bone formation and the prevention of bone resorption with slight side effect. Genistein provides an additional viable way to therapy for osteoporosis in the jaw bones.