摘要
目的 建立血管瘤动物模型 ,进一步探讨血管瘤发生机制 ,筛选治疗方法。方法 构建并鉴定PyMT基因 ,应用DNA显微注射法 ,将目的基因导入小鼠受精卵 ,并植入母鼠输卵管。观察转基因小鼠表型。分子生物学方法 (PCR法 )检测目的基因整合情况。组织学检查转基因小鼠可疑血管瘤样新生物。结果 目的基因经酶切、鉴定 ,证实大小无误。DNA显微注射 ,移植卵 5 79枚 ,产仔 6 2只。小鼠表型观察 :6 2只小鼠中 1只出现血管瘤样表型 ,主要位于皮肤、舌、胃等组织器官的黏膜表面。转基因小鼠出现的血管瘤样新生物经病理诊断证实为异常血管增生 ,类似海绵状血管瘤结构。PCR方法检测证实该小鼠体内有PyMT基因表达。结论 PyMT基因整合到小鼠基因组中可导致血管瘤形成 ,形成的血管瘤主要分布于皮肤及黏膜组织丰富的器官。
Objective To set up the animal model of hemangioma by microinjecting the PyMT transgenic DNA. Methods Constructing the transgenic PyMT gene, and microinjecting it into fertilized embryos which were transferred to pseudopregnant recipients then. Observing the phenotype of the newborn-mice, detecting the integration of transgenic DNA by PCR, and analyzing the histological morphon of the neoplasm of the mice. Results The transgenic DNA was proved to be right and has been microinjected into 579 fertilized embryos, 62 mice were born. Within the 62 mice, one mouse was found being the phenotype of henangioma. PyMT gene was expressed in the total DNA of the transgenic mouse by PCR. ConclusionIt could be a good way to build animal model of hemangioma with transgenic PyMT DNA.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2003年第5期355-357,I002,共4页
Chinese Journal of Stomatology
基金
上海市科技发展基金资助项目 (990 8190 91)