人牙周膜细胞在弹性牵张力作用下核心结合因子mRNA的表达

Effect of elastic stress on expression of corebinding factor a 1 mRNA in human periodontal ligament cells in vitro

目的 探讨人牙周膜细胞在应力作用下的成骨特性及核心结合因子 (corebindingfactora 1,cbfa1)在正畸成骨和正畸牙齿移动中的作用机制。方法 体外原代培养新生SD大鼠颅骨成骨细胞 ,进行大鼠cbfa1cDNA片段的克隆和序列测定 ,制备大鼠cbfa1cDNA探针。人牙周膜细胞在弹性膜上体外培养 ,采用体外培养细胞加载系统加载弹性牵张力。用原位杂交方法检测cbfa1mRNA的表达。结果 正常对照组不加力的人牙周膜细胞未检测到cbfa1mRNA阳性表达 ,实验组加载弹性牵张力的人牙周膜细胞出现cbfa1mRNA阳性表达。结论 弹性牵张力可以诱导人牙周膜细胞向成骨样细胞分化 ;

Objective To investigate the osteoblast-like characteristics of human periodontal ligament cells affected by elastic stress in vitro, and the role of corebinding factor a 1 (cbfa1) in alveolar bone formation during orthodontic tooth movement. Methods Rat dig-labeled cbfa1 cDNA probe was prepared from SD rat osteoblasts cultured in vitro. Human periodontal ligament cells were cultured on the elastic bottom plate and stimulated by elastic stress using mechanical loading system for cultured cells in vitro. The expression of cbfa1 mRNA was detected by in situ hybridization method. Results Cbfa1 mRNA express in human periodontal ligament cells stimulated by elastic stress and did not express in normal human periodontal ligament cells. Conclusion It is suggested that elastic stress plays a role in the differentiation process from human periodontal ligament cells to osteoblast-like cells. Cbfa1 is a transcription factor in alveolar bone remodeling during orthodontic tooth movement.