干扰素α-2b治疗准分子激光角膜切削术后角膜上皮下混浊的实验研究

Interferon α-2b inhibits rabbit corneal haze induced by PRK

目的 了解干扰素α 2b对PRK术后兔眼角膜上皮下混浊 (Haze)的抑制作用。方法将 36只家兔随机均分成A、B、C 3个组 ,分别作为实验组、对照组及空白对照组。每组分别按照屈光度数为 - 4 0 0、- 6 0 0、- 8 0 0及 - 10 0 0D各切削 3只兔 ,术后 3个组分别滴用 1× 10 5IU/ml干扰素α 2b、0 1%地塞米松及生理盐水 2个月。术后检查Haze情况和眼压 ,观察有无并发症。并于术后 30、45及 6 0d时分别处死每组不同屈光度数的 1只兔进行角膜组织病理学检查。结果 术后A、B组Haze发生情况及病理改变相近 ,而C组Haze情况较A、C组严重 ,且术后活跃的角膜上皮下纤维增生在术后 6 0d时仍然存在。A组术后眼压与术前相比 ,差异无显著意义 (P >0 0 5 ) ,B组术后眼压则明显高于术前。结论 1× 10 5IU/ml干扰素α 2b对兔眼PRK术后Haze的抑制效果与 0 1%地塞米松相当 ,但无明显毒副作用。

Objectives To evaluate the effect of interferon α-2b on the treatment of corneal haze after PRK. Methods 36 rabbits were divided into three groups(A.B.C).The refractive degree of ablation was -4.00 D,-8.00 D,-10.00 D in each group respectivily. The treatment given in group A was 1×10 5 IU/ml interferon α-2b, in group B 0.1% dexamethsone and placebo eyedrops for group C after PRK 2 months. Corneal haze, histopathologic response, intraocular pressure (IOP) were investigated. Results Less corneal haze was observed in group A and B. Postoperative IOP was higher than that of preoperation in group B. Keratocytes and fibrotic response in corneal stroma were active up to 2 months in group C. Conclusions Interferon α-2b was effective on inhibition of corneal haze after PRK.