肿瘤坏死因子(TNF)对流行性出血热、甲型肝炎病毒的作用研究

Studies on the Effects of rhTNF-α Anti-repli-cation against EHFV and HAV In Vitro

流行性出血热和甲型肝炎严重危害人民健康。目前对流行性出血热病毒和甲肝病毒还暂无特效药物,近年报道肿瘤坏死因子具有抗病毒活性,本文报告用在国内分离、克隆并表达的人重组肿瘤坏死因子(rhTNF-2)在体外对流行性出血热病毒及甲型肝炎病毒的抗感染作用进行了研究,现将结果报告如下。

It was demonstrated in this study that rhTNF-a can inhibit the replication of epidemic hemorrhagic fever virus and hepatitis A virus in tissue culture. EHFV infection produced strong fluorescence (+++～++++) on Vero-E_6 cells after 9 days. In the rhTNF-a treated group, however the fluorescence was very weak (-or±). rhTNF-a also showed anti-HAV effects on 2BS cells. The fluorescence intensity on 2BS cells infected by HAV was +++～++++, but in the rhTNF-a treated group, the fluorescence was negative. We observed that rhTNF-a inhibited EHFV antigen expression on Vero-E_6 at different times (-2h, 0h, +2h). Dr. Old had recently suggested that the physiological role of rhTNF-a is that of a protective agent against infectious diseases, and this view is supported by our findings.