老化抑制阿片肽增强大鼠T淋巴细胞转化和阿片肽增强小鼠巨噬细胞的吞噬作用

Ageing Suppresses the Enhancement of T Cell Mitogenesis by Opioid Peptides and Enkephalins Increase Phagocytosis of Murines Macrophage

阿片肽对老龄动物脾淋巴细胞丝裂原反应性的影响,迄今作者只检索到1篇文献(1988),该文作者观察到老龄可降低β-内啡肽(β-endorphin,β-END)增强丝裂原诱导的细胞增殖作用。本实验选用不同浓度的甲硫脑啡肽(methionine enkephalin,MENK)和β-END,分别作用于PHA激活的老龄Wistar大鼠脾淋巴细胞,探索两类阿片肽对老龄大鼠脾淋巴细胞的作用,观察年龄对中枢神经内分泌调节免疫功能的影响。此外,本实验还观察阿片肽对巨噬细胞吞噬作用的影响,以便全面了解阿片肽和免疫功能的关系。

The opioid peptides methionine enkephalin (M-ENK) and β-endorphin (β-END) (1× 10-6-1×10-11mg/ml) were investigated for their effect on the PHA (1:500, 1:750 or 1:1000) induced proliferative response of old and young Wistar rat splenic lymphocytes in vitro. The different effects in young and old rats on proliferative response to PHA were determined. The results showed that MENK and β-END significantly enhanced the proliferative response to PHA in young rats, while enhancement by M-ENK and β-endorphin (PHA 1:750, 1:1000) was not observed in old rats. The PHA-induced proliferative response was 30%-40% lower in old rats than in young rats.Our results suggest an altered response to neuroimmunomodulation with age.The in vitro effect of ENKs on phagocytosis was also studied. The results indicated that LENK(10-4-10-6mg/ml) and MENK (10-2-10-4mg/ml) could stimulate the phagocytosis of peritoneal macrophages from Balb/c mice.