过碘酸-石炭酸副品红染色对某些真菌病及分枝杆菌病的诊断价值

Evaluation of PA-CPR staining in the diagnosis of fungal and mycobacterial dermatoses

目的:探讨过碘酸-石炭酸副品红(PA-CPR)染色对某些真菌病及分枝杆菌病的诊断价值。方法:采用PA-CPR染色法对皮肤淋巴性感染的皮肤活检标本染色,进行组织病理学和微生物学检查。结果:3例过碘酸复红(PAS)染色和抗酸(AF)染色阴性标本,采用PA-CPR染色2例查见真菌,1例查见分枝杆菌。结论:PA-CPR染色法可以替代PAS染色和AF染色,用于真菌病及分枝杆菌病的初步定性诊断和鉴别诊断。

Objective:To evaluate periodic acid-carbol pararosanilin allochrome (PA-CPR)staining in the diagnosis and dif-ferential diagnosis of lymphocutaneous infections of fungus and mycobacterium.Methods:The biopsies taken from the skin lesions were stained with PA-CPR and then examined histopathologically and microbiologically.Results:In this study,three negative samples with periodic acid-Schiff(PAS)and acid-fast(AF)staining were proved to be positive after stained again with PA-CPR,with fungi detected in two and mycobacte ria found in one.Conclusions:PA-CPR staining is a simple and cost-effective screen ing method for the diagnosis and differential diagnosis of lymphocutaneous infections of fungus and my -cobacterium.