应用流式细胞术对胸腺瘤恶性程度的分析

Grading of Malignancy of Thymomas by Flow Cytometry

<正> 应用流式细胞术(flow cytometry,FCM)分析新鲜组织或培养细胞DNA含量已有多年历史。FCM分析肿瘤细胞DNA含量,在肿瘤诊断,预后的预测和治疗反应上有重要价值。自1983年Hedley~[1]等用流式细胞术分析常规固定,石蜡包埋组织DNA含量检测方法建立后,已有许多学者运用此方法分析良性或恶性实性肿瘤,对病理标本材料进行了回顾性分析,如对乳腺、卵巢、结肠、直肠、肾及肾上腺,膀胱,子宫肿瘤及非何杰金氏恶性淋巴等瘤。而对胸腺瘤的分析报道很少。本研究采用胸腺瘤石蜡包埋组织材料,应用DNA流式细胞技术,对胸腺瘤恶性程度进行分析。

The nuclear DNA contents of paraffin embedded archival tisses from 33 cases of thymoma and carcinoma of the thymus were studied by flow cytometry (FCM). Twelve cases were ruled out because of coefficients of variance (CV) >10.0, leaving 21 cases for analysis. Included were 9 cases of non-invasive thymomas and 12 cases of invasive thymomas and thymic carcinomas. Among non-invasive thymomas, the DNA content was diploid in 4 cases and aneuploid in 5 cases. The DNA content was aneuploid in all 12 cases of invasive thymomas and thymic carcinomas. Proliferative activity, determined by measuring S phase percentage and proliferative index, was significantly increased in invasive thymomas and thymic carcinomas as compared with the non-invasive ones. The average S phase prec.entage for invasive thymomas and thymic carcinomas was 29.17 ± 6.91, and for non-invasive thymomas it was 15.86±3.17 (P<0.001), The average proliferative indices in the invasive and non-invasive groups were 47.76±5.03 and 32.58±6.58, respectively (P<0.001).