溃疡性结肠炎患者肠粘膜中趋化因子和核转录因子的变化

Changes of chemokine and nuclear factorκB in intestinal mucosa of patients with ulcerative colitis

目的 :研究溃疡性结肠炎 (UC)患者肠粘膜中趋化因子IL 8,MCP 1和核转录因子NF κB的变化 .方法 :患者30例经柳氮磺胺吡啶和糖皮质激素治疗前后与正常人比较 .RT PCR法半定量检测肠粘膜中IL 8,MCP 1的变化 ;采作凝胶电泳迁移率改变分析 (EMSA)法检测肠粘膜细胞核内核转录因子NF κB的变化 .结果 :UC急性期IL 8/G3PDH的比率为 1.0 9± 0 .2 2 ,缓解期为 0 .4 9± 0 .2 1,与正常对照组 0 .14± 0 .10相比均有明显增加 (P <0 .0 5 ) ,治疗前后有显著差异 (P <0 .0 5 ) ;MCP 1/G3PDH的比率为急性期 0 .89± 0 .2 3,缓解期为 0 .81± 0 .19,与正常对照组 0 .0 7± 0 .0 4相比均有明显差异 (P <0 .0 1) ,但治疗前后无明显差异 .肠上皮细胞核内NF κB亦明显增加 .结论 :UC的发生发展与IL 8,MCP 1等趋化因子的变化密切相关 ,它的表达由细胞核内NF κB调控 .柳氮磺胺吡啶和糖皮质激素可能通过抑制NF κB的合成达到抗炎目的 .

AIM: To investigate the changes of chemokine and nuclear factorκB in patients with ulcerative colitis (UC). METHODS: The colon mucosal biopsy specimens were obtained from 30 UC patients treated with sulphasalazine (SASP) plus glucocorticoid. The expressions of chemokine IL 8 and MCP 1 were measured by RT PCR. The expressions of NF κB were measured by electrophoretic mobility shift assay (EMSA). RESULTS: The expressions of IL 8 and MCP 1 increased obviously at the active phase (1.09±0.22, 0.89±0.23, P <0.01) and inactive phase (0.49± 0.21, 0.81±0.19, P <0.01) compared with those of control (0.14±0.10, 0.07±0.04). The expressions of NF κB were significantly higher in active phase of UC than those of control ( P <0.01). CONCLUSION: The occurrence of UC correlates with the increase of IL 8 and MCP 1. NF κB modulates the secretion of IL 8. Sulphasalazine (SASP) and glucocorticoid inhibit inflammation by blocking the activation of NF κB.