在甲基纤维素半固体培养基上制备抗人黄体生成素McAb

Generation of Monoclonal Antibodies (McAbs) Against Human Luteinizing Hormone (hLH) in a Methyceliulose Semi-solid Medium

自Khler和Milstein创建单克隆抗体(McAb)技术以来.该技术不断发展完善,其应用亦日益广泛。人黄体生成素(hLH)是脑垂体前叶促性腺细胞分泌的一种糖蛋白激素,是调节男女生殖机能的重要物质。目前,国外一些实验室已建成了hLH的单克隆抗体细胞株,并应用于临床诊断,但国内尚未见报道。本研究旨在建立自己的hLH McAb细胞株,以满足国内临床和计划生育科研工作的需要。

McAbs against hLH were generated by a facile hybridoma technique according to the methods of Davis and Lee, et al. After routine immunization and cell fusion, the cells were plated in a semi-solid medium containing methycel-lulose and HAT. Five to seven days later, visible clones were removed for subculture in DMEM liquid medium containing 15% FCS and then screened by ELISA. As a result, 12 hybrid cell lines secreting McAbs to the LH-B subunit were obtained. These Abs have been used in clinical diagnostic and immunohis-tochemical work, showing good results.The advantages of the semi-solid medium in preparing McAbs are discussed.