摘要
本文目的在于探索骨髓基质细胞源因子-1(SDF-1)的功能性受体在非洲爪蟾胚胎发育过程中的表达状况,进而探讨SDF-1在胚胎发育中的作用。采用胚胎细胞分散培养及钙成像技术对不同发育时期及胚胎不同部位的分散培养细胞进行了检测,结果证明:在主要由神经板、神经嵴细胞和基板迁移而来的胚胎头部组织中,其功能性受体表达量远高于背部及尾部组织(P<0.001),并从胚胎14～21期呈上升趋势,在21～35期保持高表达率。结果提示,此功能性受体在胚胎期的表达密度与成体相应区域的器官结构复杂程度呈正相关,说明SDF-1及其受体在爪蟾的生长发育中起作用。
To explore the expression profile of functional receptor of strornal cell-derived factor 1 (SDF-1) in the development of Xenopus Laevis and to inquire the function of the receptor in early development, cultured cells from different parts of embryo and from different stages were examined by calcium imaging technique. The results verified that the expression of functional receptor of SDF-1 in head tissues which derived mainly from neural plate, neural crest cells and placodes was higher than that in the dorsal and tail of embryo (P<0. 001). The expression in head increased from stage 14 to 21 and stayed at high level from stage 21 to 35. The results showed that the expression density of functional receptor of SDF-1 was positively correlated with the complexity of certain organs of adult ones, which indicates that SDF-1 and its receptor may play a role in the development of Xenopus Laevis.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2003年第1期1-5,共5页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(No.30070245)资助项目
