人宫颈鳞状上皮细胞癌克隆细胞系的研究 Ⅱ.HGPRT-突变型细胞系的建立及其生物学特性

Studies on Human Cervical Carcinoma Cell Line Ⅱ. Establishment of HGPRT-Cell Line CC-80IAR_2 and Its Biological Characteristics

用具有稳定遗传标记的突变型细胞进行体细胞杂交的技术,已被广泛应用。突变型细胞形式多样,次黄嘌呤鸟嘌呤磷酸核糖转移酶缺失(HGPRT-)的突变体细胞是常用的杂交亲本细胞类型。杂种细胞易于在HAT(含次黄嘌呤、鸟嘌呤、胸腺嘧啶核苷)培养液中被筛选。本研究用N-甲基-N-硝基-N-亚硝基胍(MNNG)处理人宫颈鳞状上皮细胞癌克隆细胞系CC-801,诱发细胞突变,然后用8-杂氮鸟嘌呤(8-AG)进行筛选,成功地分离了几株抗高浓度8-AG的突变细胞株。

An HGPRT- cell line, CC-801 AR2, was established from cloned human cervical carcinoma cell line CC-801 by using MNNG to induce cell mutation and 8-azaguanine (8-AG) to select the 8-AG resistant cells. The deficiency of HG-PRT in CO-801AR2 cells was proved by enzyme activity assay. This cell line was very sensitive to HAT medium. It was very similar in biological characteristics to CC-801, especially in that it retained its tumorigenicity when transplanted into nude mice. CC-801 AR2 can therefore be used in somatic cell hybridization and gene transfer experiments.