柔红霉素生物合成的研究

ADVANCES IN BIOSYNTHESIS OF DAUNOMYCIN

根据近十余年来柔红霉素生物合成机制及其有关研究的进展,结合作者的研究工作,对其生物合成途径作了评述。

<Absrtact>Using the mutants, SIPI 80334-U33,from Xiangfanmycin-producing strain S1PI 80$94, and SIPI 1482-NS-4 from daunomycin-producing strain SIPI 1482; daunomycinone and daunomycin were transfered into 13S-and 13 R-dihydrodaunomyci-nones, dihydrodaunomycin and its N-acstyi derive.tive, N-acetyl-daunomycin, respectively. The cha-racteristics of biotranatormation of strains of dif-ferent genus were also discussed.Several compounds produced in the fermenta-tion of SIPI 1482 were identified, and the biosyn.thetic pathway of daunomycin was proposed, ac.cording to the changes of various compounds and the fermentation test of addition certain erogenous substances. The medium components of pH, temperature and aeration could affect tho bi}ynthesis of dau-nomycin and the formation of by-products inclu-ding the conversion f.E-rhodomycinone to glycosi.des. Daunomyein would be increased and with less by-products if the strain was cultivated in the medium containing 7 } of corn meal.The mutant SIPI 1482-NS-4, producing no dau.nomycin in Cause I medium, rescovered its ability of daunomycin synthesis in the medium contain.ing corn meal or its hydrolysate. In the fermen-tation of SIPI 1482 and SIPI PF-25 in mediumcontaining 7% corn meal and of its barolysate,the production of daunomycin increased morethan 10 and 30 respectively.