Smad7反义寡核苷酸对TGF-β1调控人牙髓细胞生长和分化的影响

The effect of antisense Smad7 oligonucleotide on the growth and differentiation of human dental pulp cells regulated by TGF-β1

目的 :观察Smad7反义寡核苷酸对转化生长因子 β1(transforminggrowthfactor -β ,TGF -β1)调控人牙髓细胞生长和分化的影响。方法 :细胞培养 ,细胞转染 ,MTT比色测定法和碱性磷酸酶活性测定法。结果 :Smad7反义寡核苷酸促进TGF -β1对人牙髓细胞增殖的诱导作用和对碱性磷酸酶活性的抑制作用。 结论 :Smad7参与介导TGF -β1对人牙髓细胞增殖和ALP活性的调控 ,提示TGF

Objective:To investigate the effect of Smad7 antisense oligonucleotide (ODN) on growth and differentiation of human dental pulp cells regulated by transforming growth factor-β1. Method: Cell culture, cell transfection, MTT colorimetric assay and assay of alkaline phosphatase (ALP) activity were used.Result: Smad7 antisense ODN promote the proliferation and ALP activity of human dental pulp cells mediated by TGF-β1.Conclusion: Smad7 mediate the proliferation and ALP activity of human dental pulp cells regulated by TGF-β1. TGF-β1 may regulate the growth and differentiation of human dental pulp cells through Smad signaling.