组培条件对何首乌愈伤组织诱导及生长的影响

EFFECTS OF CULTURE CONDITIONS ON CALLUS INDUCTIONAND GROWTH OF POLYGNOUM MULTIFLORUM

为了寻找适合何首乌愈伤组织诱导与生长的培养条件,本文考察了愈伤组织形成的比率,分析了取样时间、光照条件、培养基中2,4-D、6BA和IBA的浓度对愈伤组织诱导和生长的影响.实验表明,在黑暗中,在添加有1.5mg L2,4-D,1mg L6BA和0.5mg LIBA的MS培养基上,夏季取样的茎段,愈伤组织诱导率可达100%.

To seek culture conditions suitable for the callus induction and growth of Polygnoum multiflorum Thunb., effects of sampling times, illumination and 2,4-D, 6BA and IBA concentration in medium were studied by examining callus formation rate. 100% of the stem segments of Polygnoum multiflorum Thunb. sampled in summer formed calli very well when they were cultured in dark on MS medium supplemented with 1.5 mg/L 2,4-D, 1 mg/L 6BA and 0.5 mg/L IBA.