摘要
目的探讨鸦胆子油乳对膀胱癌BIU 87细胞坏死与凋亡的影响及其可能的作用机制。方法将不同浓度的鸦胆子油乳加入体外培养的BIU 87细胞 ,用流式细胞仪检测细胞凋亡与坏死比例、并检测线粒体膜电位 (MMP) ,用激光共聚焦显微镜观察细胞色素C分布。结果高浓度鸦胆子油乳作用 4h后 ,肿瘤细胞MMP明显降低 ,2 4h后多数细胞发生坏死 ,与对照组相比有非常显著性差异 (P <0 0 1) ;低浓度鸦胆子油乳作用 6h后 ,诱导细胞色素C释放 ,2 4h后可见典型的凋亡现象 ,与对照组相比有显著性差异 (P <0 0 5 )。结论鸦胆子油乳高浓度主要诱导肿瘤细胞坏死 ,低浓度诱导细胞凋亡。
Objective To study the effcet of necrosis and apoptosis induced by Brucea javanica oil emulsion on bladder cancer BIU-87 cells. MethodsBrucea javanica oil emulsion of different concentration was added to the bladder cancer BIU-87 cell cultured in vitro. The ratio of apoptosis/necrosis cells and mitochondrial membrane potential(MMP) were detected with flow cytometer. The dissipation of cytochrome C was observed with confocal microscopy. ResultsHigh concentration brucea javanica oil emulsion reduced MMP which led cell necrosis, while that of low concentration diffused the cytochrome C from mitochondria to cytoplasm which induced cell apoptosis. ConclusionHigh concentration brucea javanica oil emulsion mainly causes BIU-87 cells to necrosis, low concentration induces cell to apoptosis.
出处
《中国康复理论与实践》
CSCD
2004年第3期163-164,F003,共3页
Chinese Journal of Rehabilitation Theory and Practice
基金
陕西省发展基金 (2 0 0 2K1 0 G4)
关键词
鸦胆子
膀胱肿瘤
坏死
凋亡
线粒体
细胞色素C
brucea javanica
bladder neoplasm
necrosis
apoptosis
mitochondria
cytochrome C
