兰州百合器官离体培养外植体位置效应观察

Position Effect on the Propagation in vitro of Different Explants from Lilium davidii var.unicolor

探讨兰州百合 (Liliumdavidiivar.unicolor)鳞茎鳞片、叶片和根的不同切段的培养效应。结果表明 :其切段不定芽的分化速度和数量是下段 >中段 >上段。芽的诱导和增殖的最适外植体为鳞茎鳞片 ,兰州百合离体培养中鳞片不定芽诱导和快速繁殖的培养基为MS +BA2mg L +NAA 0 2mg L ,增殖培养基与诱导培养基相同 ,3周左右不定芽开始分化。叶和根不同部位中不定芽的发育能力大体与鳞茎鳞片一致 ,但低于鳞片 ,较适宜的培养基为MS +BA2mg L +NAA 0 4mg L ,生根培养基为 1 2MS +NAA 0 3mg L ,约 15d生根 ,生根率大于95 %。月增殖率为 1∶4 ,整个繁殖周期约需 3个月。

The propagation in vitro of Lilium davidii var.unicolor were conducted with different parts of scales,leaves and roots respectively as explants.The results revealed that the proliferation speed and differentiation quantity of adventitious shoots were as follows:lower part>median part>upper part.The most suitable explants for multiple shoot differentiation and rapid growth in vitro propagation of lily is bulb-scale,on the media of MS+BA 2?mg/L+NAA 0.2?mg/L.Media for proliferation of leaf and root was the same as for shoot bulb-scale,and differentiation occurred in three weeks.The developmental potentialities of adventitious shoots in different parts of leaf and root are in accordance with bulb-scale,though they are lower than that of bulb-scale.Roots were induced from buds on the media of 1/2 MS+NAA 0.3?mg/L.The rooting rate could be over 95%.The proliferation rate reached 1∶4 per month.The whole propagation cycle was about three months.