狼疮性肾炎外周血淋巴细胞Fas、FasL、Bcl-2表达与血瘀证积分的关系

The relationship between expression of peripheral blood lymphocyte Fas、FasL、Bcl-2 and measure of blood stasis syndrome in lupus nephritis

探讨外周血淋巴细胞 ( PBL)凋亡调控因子和血瘀证积分的关系及其临床意义。对 40例活动性狼疮性肾炎 ( LN)患者 PBL细胞凋亡调控因子 Fas抗原、Fas配体、Bcl-2免疫组化法检测分析及评估血瘀证积分。结果显示 :均符合血瘀证判断标准 ,说明活动期 LN患者大多有血瘀病理因素的存在 ;血瘀证积分和 Fas、Fas L、Bcl-2的表达 ,ANA、抗 ds-DNA、病理类型的 Spearman等级相关分析均呈正相关 ( P<0 .0 1 ) ,与 Fas表达最密切 ( r=0 .90 3 )。说明随着 Fas、Fas L、Bcl-2的表达增多 ,ANA、抗 ds-DNA滴度升高 ,血瘀病理改变越趋严重 ;也说明血瘀病理因素改变程度可反映患者肾损害病理类型。

To investigate the relationship and its clinical value between peripheral blood lymphocyte (PBL)apoptosis regulating factors and measure of blood stasis .The PBL apoptosis regulating factors antigen Fas?ligand Fas?bcl-2 were assayed and analysised by immunohistochemical method and the blood stasis accumulated points were evaluated in 40 cases active LN patients.The result shows that they accord with the judging standard of blood stasis syndrome so that the pathologic factors of blood stasis exist in most active phase LN patients.The blood stasis accumulated points have positive correlation with Fas?FasL?bcl-2 expression?ANA?antids-DNA and pathological Spearman ranks correlation analysis(P<0.01),and it has the closest relationship with Fas expression(r=0.903),indicates the pathological change of blood stasis getting more and more Serious together with the increasing of Fas?FasL?bcl-2 expression and the tite of ANA?antids-DNA;It also shows that the grade of the blood stasis pathologic factor changing can reflect the type of kidney impairment in patients.