白细胞介素-10对大鼠脑缺血的保护作用

The study of the protective mechanism of interleukin-10 on cerebral ischemia in rats

目的 探讨白细胞介素(IL)-10对大鼠脑缺血保护作用的机制。 方法 采用改良Zea Longa线栓法制作大鼠大脑中动脉栓塞模型(MCAO)。将健康成年大鼠随机分组原则分成以下4组:IL-10干预组、等渗盐水对照组、单纯缺血组和假手术组,每组8只。干预组在缺血3 h经侧脑室注射1μg人工重组IL-10,对照组注射5μl等渗盐水。单纯缺血组和假手术组不作干预治疗。24 h后观察血清和脑组织中一氧化氮、丙二醛、超氧化物歧化酶(SOD)和一氧化氮合酶(NOS)的变化。 结果 应用IL-10组大鼠脑组织中一氧化氮、丙二醛、SOD、内皮型NOS(eNOS)、诱导型NOS(iNOS)和神经元型NOS(nNos)分别为(2.12±0.35)μmol／g、(2.95±0.99)μmol／g、(248±21)×103IU／g、(0.81±0.21)×103IU／g、(0.52±0.20)×103IU／g和(1.5±0.4)×103IU／g,与等渗盐水组大鼠比较iNOS、丙二醛和一氧化氮明显降低,SOD明显升高(P<0.05),而eNOS和nNOS变化不明显(P>0.05)。应用IL-10大鼠组血清中一氧化氮、丙二醛、SOD、eNOS、iNOS和nNOS分别为(35±15)μmol／g、(12.9±1.5)μmol／g、(316±33)×103IU／g、(1.1±0.3)×103IU／g、(0.8±0.4)×103IU／g及(2.1±0.7)×103IU／g,与等渗盐水组大鼠比较差异无显著意义(P>0.05)。无论大鼠脑组织还是血清中一氧化氮、

Objective To study the protective mechanism of interleukin-10 treating cerebral ischemia in rats. Methods The middle cerebral artery (MCA) was occluded with thread according to modified Zea Longa's method. The rats were randomly divided into four groups: the interleukin-10 group, the saline control group, the operation group and the sham operation group. The rats of the interleukin-10 group were administered with 1μg IL-10 centrally into lateral ventricle 3 hours after operation; the rats of the saline control group were administered with 5μl saline. The rats of the sham operation group and the operation group were not administered with IL-10 or saline. After 24 hours measured the contents of NO, MDA, and the activities of iNOS, eNOS, SOD and nNOS in the brain tissue and the serum. Results In the brain tissue of IL-10 group the content of NO was (2. 12±0. 35)μmol/g, MDA was (2. 95±0. 99)μmol/g, the activities of SOD, eNOS, iNOS and nNOS were (248±21)×103 IU/g, (0. 81±0. 21)×103IU/g, (0. 52±0. 20)×103 IU/g and (1. 5±0. 4)×103 IU/g respectively, compared with the saline group the contents of NO, MDA, and the activity of iNOS decreased significantly, the activity of SOD increased significantly ( P<0. 05) . The activities of eNOS and nNOS didn't show significant difference ( P> 0.05) ; In the serum the content of NO was (35±15)μmol/g, MDA was (12. 9±1. 5) μmol/g, the activities of SOD, eNOS, iNOS and nNOS were (316±33)× 103 IU/g, (1. 1±0. 3)×103 IU/g, (0. 8±0. 4)×103 IU/g and 2. 1±0. 7×103IU/g respectively, compared with the saline group didn't show significant difference ( P>0. 05) . Compared with the sham operation group the contents of NO , MDA , and the activities of iNOS, eNOS and nNOS increased significantly, the activity of SOD decreased in the focal brain and serum ( P>0. 05) . Conclusion The results indicate that IL-10 can alleviate the injury of cerebral ischemia. The decrease of NO content and iNOS activity and the increase of SOD activity after ischemia may all contribute to the protective effect.