地塞米松对小鼠H_(22)肿瘤生长及血管生成的抑制作用

Inhibition of Dexamethasone to Tumor Growth and Angiogenesis in Mouse H_(22) Model

目的 探讨地塞米松对小鼠H2 2 肿瘤生长及血管生成的影响。方法 BALB c小鼠皮下接种H2 2 细胞 ,分别从接种当天和第四天开始给药 ,每天测肿瘤直径。第 12天处死动物 ,称瘤重。用抗CD31单抗对肿瘤组织做免疫组化微血管计数及流式细胞分析CD31表达。结果 地塞米松对小鼠H2 2 肿瘤的生长有显著的抑制作用 ,P<0 0 5。两给药组的瘤重和最终瘤体积与对照组比较均显著减小 ,P值均 <0 0 5 ,按瘤重计算 ,接种当天和第四天开始给药组的抑瘤率分别为 31 4 8%和 35 38%。从免疫组化及流式细胞分析的结果上看 ,接种当天给药组的微血管数比对照组少 ,差异有显著意义 ,P <0 0 1,接种第四天开始给药组的微血管数与对照组相比虽有减少 ,但无显著差异。结论 地塞米松对小鼠H2 2 肿瘤生长有显著的抑制作用。地塞米松的抗肿瘤效果和抗血管生成作用有一定的相关性 ,但对已形成的肿瘤 。

Objective To observe the effect of dexamethasone on tumor growth and angiogenesis in mouse H 22 model. Methods H 22 cells were injected subcutaneously into BALB/c mice. Drug treatment began from the day of inoculation (first group) or from the fourth day after inoculation (second group). Tumor's diameters were measured every day. In the twelfth day mice were sacrificed and tumors were weighed. Tumor microvessel count was performed by immunohistochemical staining and flow cytometry assay with anti-CD31 monoclonal antibody. Results Tumor growth was inhibited by dexamethasone significantly in both groups. Both tumor weight and final tumor volume of the treatment were smaller than the control, P<0.05, and the rates of inhibition were 31.48% and 35.38% in the first and second group respectively. In first group, the tumor microvessel count decreased significantly vs control, P<0.01; in the second group, tumor microvessel count was reduced too, but it had no markedly difference compared with control. Conclusions Dexamethasone inhibits tumor growth in mouse H 22 model significantly. The inhibition may be correlated with the anti-angiogenic effect of dexamethasone, but when tumor has already grown to certain size, the efficacy is limited.