慢性再生障碍性贫血患者外周血T淋巴细胞亚群改变与中医分型的关系

The Relationship of T-Lymphocyte Subsets and Symptom Complex Groups of Patients with Chronic Aplastic Anemia

用McAb和ABC法测定了38例慢性再生障碍性贫血(CAA)患者外周血T淋巴细胞亚群(简称T亚群)。与健康人相比,CAA的Ts明显增高(P<0.001),Th/T5明显降低(P<0.001)。并发现外周血Hb水平、病程、疗效及中医证型与T亚群相关。按气血两虚肾阳虚、肾阴虚、肾阴阳两虚的顺序,患者Th逐渐降低,Ts逐渐升高,Th/Ts明显降低。后两组比前两组Th/Ts下降更显著(P<0.01),提示阳损及阴时机体免疫机能发生更大的改变,从T亚群水平揭示了中医证型特别是肾阳虚、肾阴虚的内涵。

The T-lymphocyte subsets of 38 patients with chronic aplastic anemia (CAA) were measured by monoclonal antibody (McAb) and ABC method and the relationship of T-lymphocyte subsets and symptom complex groups by traditional Chinese medicine theory were also analysed. It was shown that the T subsets of patients with CAA have remarkable changes:Ts (41.3±11.2%) increased more than the control (22.9±4.5%), Th/Ts decreased significantly more than the normal control (0.98±0.38 to 1.59±0.38). From the group of deficiency of both vital energy and blood to the group of deficiency of Kidney-yang(阳) to the group of deficiency of Kidney-Yin(阴) to the group of deficiency of both Kidney-Yang and Kidney-Yin, the Th lymphocyte gradually decreased, Ts lymphocyte successfully increased (from 33.8±7.9% to 57.7±7.5%), Th/Ts ratio declined (from 1.29±0.36 to 0.57±0.19). The Th/Ts ratio of the latter two groups decreased more than the former two groups (P<0.01). These results indicated that the T lymphocyte subsets and symptom complex group of patientswith CAA were closely related and when deficient Yang affects Yin the immunologic function of body has a more obvious change. This revealed the cannotation of Kidney-Yin and Kidney-Yang on the immuno-regulating cells (T lymphocyte subsets) level.