黄连对大鼠脂质过氧化及抗氧化酶活性的影响

The Effect of Coptis Chinensis on Lipid Peroxidation and Antioxidases Activity of Rats

本实验观察了黄连对大鼠脑匀浆(体外)生成脂质过氧化物的影响及对四氧嘧啶所致大鼠脂质过氧化的影响结果:(1)5%的黄连使大鼠脑匀浆丙二醛(MDA)的生成量极明显少于对照组(P<0.001)。(2)黄连组的大鼠胰、肝脏中的MDA含量较空白对照组及四氧嘧啶组均显著降低(P<0.01,<0.05);各组大鼠红细胞中的SOD活性比较无显著性差异;四氧嘧啶组大鼠血液过氧化氢酶活性较空白对照组有明显下降(P<0.05),但黄连组与四氧嘧啶组比较无明显恢复;四氧嘧啶组血糖较空白对照组有明显升高(P<0.05),黄连组与四氧嘧啶组比较有降血糖趋势,但无显著性差异(P>0.05)。

In order to make a systematic study of the effect of Coptis chinensis on free radicals, the authors used the method that the drug and the brain homogenate of rat were mixed and incubated to investigate the effect of Coptis on lipid peroxidation. The result showed that the malondialdehyde (MDA) product of rat brain homogenate inhibited by 5% Coptis was significantly different from control (P<0.001). On the basis of the above-mentioned results, the effect of Coptis on lipid peroxidation and diabetes of rats induced by alloxan was investigated. The result Showed: (1) The MDA product of both pancreas and liver homogenate in Coptis group was significantly less than that in control and alloxan group (P<0.01, P<0.05). (2) Superoxide dismutases (SODs) in erythrocytes activity was the same for all groups(P>0.50). (3) The blood catalase (CAT) activity in alloxan group markedly decreased compared with control group (P<0.05), but no significant change between Coptis and alloxan group (P>0.05). (4) The value of serum glucose in alloxan group was significantly increased in comparing with control groop (P<0.05). There was a trend to decrease the value of serum glucose in Coptis group compared with alloxan group, but no significant difference between two groups (P>0.05). The experiment indicated that there was very strong inhibitory effect of Coptis to the lipid peroxidation in vitro and in vivo. Coptis could protect rat from diabetes inducing by alloxan and that probably was due to the fact that Coptis was able to inhibit alloxan inducing free radicals.