腹部外科血瘀证患者免疫功能状态的研究

Study of Immune Function Blood Stasis Syndrome in Patients with Abdominal Surgical Diseases

应用流式细胞术测定53例不同类型的腹部外科血瘀证患者的外周血T淋巴细胞亚群,并应用免疫法检测血清免疫球蛋白和补体水平,观察部分患者的外周血T细胞超微结构。结果表明:气滞血瘀型患者的免疫功能正常;湿热血瘀型患者的细胞免疫和体液免疫的免疫应答活跃;气虚血瘀T_(?)型患者细胞明显降低,T细胞显著升高,抗体水平亦降低明显,说明此型患者存在明显的细胞免疫及体液免疫功能抑制现象。电镜观察还发现气虚血瘀患者的T淋巴细胞有较明显的超微结构异常改变。

52 Patients with Blood Stasis Syndrome (BSS) in abdominal surgical diseases were divided into 3 types according to their symptoms, signs and natures of diseases. Peripheral blood T lymphocytesubsets of these BSS patients and 12 healthy persons were studied with Flow Cytometry andmonoclonal antibodies. The immunoglobulins and complements of these cases were also studied.There were no difference in T cell subsets, immunoglobulins and complemente between Qizhi(气滞)-BSS group and normal control. The Shire (湿热)-BSS group showed that CD_8 cell, IgG, IgM, andC_1, C_(3c) were increased. These results showed that the immune response increased in this type of BSSpatients. In the Qixu (气虚)-BSS group, the CD_3 was nearly normal, and the CD_4^+, CD_4^+/CD_8^+ ratio.and CD_(16)^+ were statistically decreased. However, the CD_8^+ cells markedly increased, the IgG, IgMand IgG were also lowered significantly in this type. The marked morphologic abnormal changes inultrastructures of T lymphocyte were found in 6 tients with Qixu-BSS group. These results showed that the Qixu type of Blood Stasis Syndrome patients were in the immuno-suppressive status.