体外培养妊娠早期人胎肝细胞对乙型肝炎病毒易感性的变化

Susceptibility change to HBV in primary culture of first trimester human fetal hepatocytes

目的 观察妊娠早期(孕10周)人胎肝细胞在不同的体外培养条件下对乙型肝炎病毒(HBV)易感性的变化,为HBV受体的发现和鉴定打下基础。 方法 设计无血清培养基,分别添加不同的细胞分化诱导物,在不同时相以HBV感染原代培养10周孕龄人胎肝细胞,检测细胞形态、功能及HBV感染标志物。 结果 培养基中添加2.5 mmol/L苯巴比妥钠,培养6d后细胞逐渐出现成熟肝细胞标志,并可被HBV感染;其他培养条件下则不能观察到HBV的成功感染。 结论 苯巴比妥钠可在体外培养时诱导早期人胎肝细胞分化,并出现HBV易感性。

Objective By culturing primary early (8 to 12-weeks-old) human fetal hepatocytes with different conditions, to study the status of cell susceptibility to HBV. Methods During primary culture of 10-weeks-old human fetal hepatocytes with serum-free medium adding different differentiation-induced ingredients, to inoculate cell with HBV at certain time. Cell shape, function and markers of HBV infection are measured. Results 6 days after seeding, markers of mature hepatocytes are observed in cells cultured with 2.5 mmol/L phenobarbital sodium, and these cells show susceptibility to HBV. Other ingredients cannot render hepatocytes susceptible to HBV. Conclusion Phenobarbital sodium induces differentiation and susceptibility to HBV in primary culture of early human fetal hepatocytes.