摘要
目的 将pCMV KAI1重组质粒转染人高转移胰腺癌细胞株MiaPaCaⅡ中 ,观察转染前后KAI1基因对胰腺癌细胞侵袭转移能力和运动能力的影响 ,从基因水平探讨KAI1基因抗胰腺癌转移机制。方法 通过脂质体转染法成功将重组质粒转染到MiaPaCaⅡ中 ,利用Transwell小室测定细胞穿膜侵袭运动能力 ,采用SDS 聚丙烯酰胺凝胶电泳检测明胶酶活性。结果 pCMV KAI1重组质粒转染后 ,显微镜下细胞计数示转染后胰腺癌细胞穿透基底膜的数量较转染前明显减少 (P <0 0 5 ) ,细胞运动能力明显减弱。聚丙烯酰胺凝胶电泳显示负染带宽度比转染前变窄 ,亮度减弱 ,降解基质膜和细胞外基质的能力和侵袭转移能力下降。结论 KAI1基因控制胰腺癌细胞转移可能是通过抑制癌细胞侵袭及运动功能来实现的。
Objective To study the effect on invasive and motor ability of MiaPaCa Ⅱ pancreatic cancer cell transfected by recombinant plasmid pCMV-KAI1,alteration of KAI1 in the trasfecting cancer cells,and the metastatic mechanism in the malignancy. Methods MiaPaCa Ⅱ pancreatic cancer cell was transfected with pCMV-KAI1 by liposome,the invasive and gelatinase ability of two pancreatic cancer cell lines were analyzed with transwell culture cell and polyacrylamided gel electrophoresis (PAGE) before and after KAI1 cDNA transfection. Results After KAI1 cDNA transfected into MiaPaCa Ⅱ,the migrative ability of passing through the membrane filte decreased evidently ( P <0.05). The width of negative staining band narrowed,the intensity level of tape weakened through PAGE. These results showed that reduced the ability of degrading basement membrane and extra-cell base and metastasis were influenced after MiaPaCa Ⅱ pancreatic cancer cells were transfected. Conclusion The suppressed invasion and motor function of pancreatic cancer cells seems to influence their metastatic ability and thereby entrances the malignant potential of pancreatic cancer.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2004年第5期360-362,共3页
Chinese Journal of Internal Medicine
基金
国家自然科学基金资助项目 (3 9970 3 44)