内淋巴积水程度的定量分析

Quantification of endolymphatic hydrops in experimental animal model

目的 探讨实验性内淋巴积水动物模型积水程度的定量分析方法。方法 30只豚鼠随机分为 3组 :对照组 10只 ,双耳未手术 ;2 0只豚鼠用右侧枕后硬脑膜外进路阻塞内淋巴囊的方法制备内淋巴积水的动物模型 ,其中术后 4、8周 2组各 10只。应用计算机辅助设计软件 (AutoCADR14 )分别测量双侧耳蜗中轴左右两侧 1～ 4回前庭阶 (scalavestibuli,SV)和中阶面积 ,计算最大中阶面积比率 ,并作比较。结果 所有未手术耳均无内淋巴积水 ,所有手术耳有不同程度的内淋巴积水。最大中阶面积比率 :术后 4周组 (2 2 2 31± 0 1996 )比对照组 (1 0 971± 0 0 6 4 4 )显著增大 ,术后 8周组 (4 0 14 2± 0 5 2 18)比术后 4周组更大 ,差异均有统计学意义 (P <0 0 5 )。结论 此测量方法方便可靠 ,可应用于定量分析内淋巴积水程度。

Objective To investigate a method of quantification of the endolymphatic hydrops in experimental animal model.Methods Thirty guinea pigs were divided into three groups at random.In control group,there were ten guinea pigs without operation on both ears.Endolymphatic hydrops was induced by endolymphatic sac obliteration through extradural posterior cranial fossa approach in right ear,including 4-week postoperative group( n =10)and 8-week postoperative group( n =10).The area of scala vestibuli(SV)and scala media(SM)of each turn on both cochlear midmodiolar sections was measured,respectively,using auto computer aided design(AutoCAD R14) software combined with digital camera,and then the maximum scala media area(SMA)ratio was calculated and compared.Results No endolymphatic hydrops was observed in all non-operated ears,however,variety degree of hydrops was present in all operated ears.The average maximum SMA ratio in the 4-week group (2.2231±0.1996) was greater than that in the control group (1.0971±0.0644).The average maximum SMA ratio of the 8-week group (4.0142±0.5218) was greater than that in the 4-weekgroup.There was significant difference between the two groups( P <0.05). Conclusion It is convenience and reliable to be used to quantify the experimental endolymphatic hydrops with the present method.This study provides a reliable methodological base for the experimental study of Meniere′s disease.