昆明山海棠离体胚培养的细胞学观察

Cytological Observation on Culture of Embryo in Vitro of Tripterygium hypoglaucum (Level.) Hutch.

首次报道了昆明山海棠离体胚的培养,诱导出愈伤组织、不定根、不定突起和丛芽,并进行了细胞学观察。指出:子叶上的愈伤组织起源于表皮及表皮下的叶肉细胞。不定根原基常来源于愈伤组织中散生分生组织或维管束。同时,对易分化形成不定根的愈伤组织的组织学特点进行了观察。

This paper for the first time reports the embryo culture in vitro of Tripterygium hypoglaucurn. Callus, adventitious roots and adventitious protuberances were induced from the cultured embryos in vitro. Multi-buds at the excised embryo tip were also observed at the same time. Cytohistological examination showed ①callus was histologically easy to be induced to form adventitious roots; ②callus on the cotyledons was originated from the epidermical and subepidermical cells of the cotyledons, and the adventitious roots were originated from the disperse meristematie tissue or vascular tissue in the callus obtained from cultured gxeised embryos.