探讨CD34^+CD44^+细胞输入量对无关脐血移植后造血恢复的影响

Effect of the number of CD34^+CD44^+ cells infusion on hematologic reconstitution after unrelated umbilical cord blood transplantation

目的 研究用无关脐血移植治疗儿童急性白血病时 ,CD3 4 + CD44+ 细胞输入量对中性粒细胞和血小板恢复时间的影响。方法 用流式细胞术分析复苏后的CD3 4 + CD44+ 细胞数 ,并对 18例儿童急性白血病患者在无关脐血移植后的中性粒细胞和血小板恢复时间等进行测定。结果 18例患者中 ,CD3 4 + CD44+ 细胞输入量为 (5.90～ 2 97.0 2 )× 10 4 /kg(中位数 54.11× 10 4 /kg)。移植后中性粒细胞 >0 .5× 10 9/L的时间为 11～ 2 3d(中位数为 17.5d) ,血小板 >2 0× 10 9/L的时间为 12～ 118d(中位数 41d)。中性粒细胞恢复时间与CD3 4 + CD44+ 细胞输入量存在相关性 ,γ值为 -0 .657,P <0 .0 1;血小板恢复时间与CD3 4 + CD44+ 细胞输入量也有相关性 ,γ值为 -0 .490 ,P <0 .0 5。结论 CD3 4 + CD44+

ObjectiveTo study whether the number of CD34 +CD44 + cells infusion influenced time to neutrophil and platelet recovery after unrelated umbilical cord blood transplan tation for the treatment of childhood acute leukemia.MethodsBy using flow cytometry, the number of CD34 +CD44 + cells was analyzed after f reeze-thawing. The clinical data of time to neutrophil and platelet recover y were also measured in 18 children with acute leukemia treated by unrelated um bilical cord blood transplantation.ResultsIn 18 cases, the number of CD34 +CD44 + cells infusion was ( 5.90 ～ 297.02 )×10 4/kg (mean 54.11 ×10 4/kg). There was a neutrophic recovery (> 0. 5 ×10 9/L, mean 17.5 days, range, 11 to 32 days), and time to platelet re covery (>20×10 9/L) was observed (mean 41 days, range 12 to 118 days). There w as the association between the number of CD34 +CD44 + cells infusion and time to neutrophic recovery (γ= -0.657 , P< 0.01 ), whereas the number of CD34 +CD44 + cells infusion correlated with time to platelet recovery (γ -0 .490 , P< 0.05 ).ConclusionThe number of CD34 +CD44 + cells infusion is involved in hematologic reconstit ution after unrelated umbilical cord blood transplantation.