紫草中萘醌的测定

Determination of Naphthoquinone in Zicao

应用薄层-比色法对不同产地紫草中5种萘醌成分进行含量测定。采取硅胶G薄层板,正己烷-丙酮-氯仿-冰醋酸为展开剂,展开前预饱和30min,在523nm波长处测定含量。

A TLC-spectrophotometric method for the separation and determination of the naphthoquinones in Zicao is described. The sample solution was applied at a point 1 cm from the bottom edge of a silica gel G plate. A mixture of n-hexane-acetone-chloroform-glaeial acetic acid was used as the developing solvent. The plate was saturated for 30 min and developed. The spots were scraped and extracted with Chloroform for spectrophotometric determination at 523nm. The method has been applied to the analysis of different samples.