喹诺酮类药物对大鼠软骨细胞体外培养的影响

Effect of quinolones on cultured chondrocytes of rat

目的 :评价 4种喹诺酮类药物 (环丙沙星、左氧氟沙星、氟罗沙星、帕珠沙星 )对幼龄大鼠软骨细胞的相对毒性 ,以及大鼠周龄、细胞培养时间对大鼠软骨细胞体外增殖和蛋白多糖分泌的影响。方法 :采用大鼠软骨细胞体外培养技术 ,将 4 ,8,12周龄大鼠的软骨细胞在各含不同药物的培养基中培养一定时间后 ,以MTT法和DMB法进行测定。结果 :4种喹诺酮类药物对4周龄大鼠软骨细胞体外增殖和蛋白多糖分泌均有抑制作用 ,其顺序为环丙沙星 >左氧氟沙星 >氟罗沙星 >帕珠沙星。环丙沙星对大鼠软骨细胞体外增殖的抑制作用随大鼠周龄的增大而减弱 ,随培养时间的延长而增强。结论 :喹诺酮类药物对大鼠软骨细胞体外培养增殖和蛋白多糖分泌具有抑制作用 ,且该作用与大鼠周龄。

AIM:To evaluate the chondrotoxicity of quinolones(ciprofloxacin,levofloxacin,fleroxacin,pazufloxacin)and the influence of animal age, treatment duration. METHODS: The chondrocytes were isolated from joint cartilage of 4,8 12-wk old rat and cultivated with various quinolones,respectively. The proliferation and the amount of proteonglycan were determined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 1,9-dimethylmethylene blue (DMB) assay. RESULTS: Order of the inhibition of proliferation and the excreation of proteonglycan was: ciprofloxacin>levofloxacin >fleroxacin>pazufloxacin. The effect of ciprofloxacin became larger with the length of duration and the increase of concentration,while became less on the chondrocytes from the older rats. CONCLUSION: Quinolones inhibited the proliferation and the excreation of proteonglycan,the effect was time-,age-dependant.