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TGFβ_1与周细胞在乳腺癌血管生成中的关系及意义 被引量：1

The relation between TGFβ_1 and pericyte in angiogenesis of breast cancer
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摘要 目的　分析TGFβ1 与周细胞在乳腺癌血管生成中的相互关系及作用。方法　取 5 0例手术切除乳腺癌新鲜标本 ,采用免疫组织化学染色、原位杂交技术 ,结合体视学分析方法 ,研究TGFβ1 及周细胞在乳腺癌中的表达情况。结果　TGFβ1 蛋白质可表达在新生血管周细胞的胞浆中 ,同时也可表达于乳腺癌癌细胞及新生血管内皮细胞 ;TGFβ1mRNA主要表达在血管周细胞的胞浆中 ,只有极少数癌细胞呈弱阳性表达。乳腺癌中TGFβ1 mRNA的表达与周细胞的表达正相关 ,P <0 0 1。结论　提示周细胞通过TGFβ1 Objective To investigate the expression of TGFβ 1 in pericyte of the angiogenesis during tumor development.Methods We study mammalian cancer samples by stereoscopy analysis, immunohistochemistry and in-situ hybridization.Results TGFβ 1 protein expressed in cancer cells, endothelium cells, pericytes of blood vessels. TGFβ 1 mRNA mainly distribute in the cytoplasm of pericyte. Stereoscopy Analysis showed that, in breast cancer, there are positive correlation between area of TGFβ 1 mRNA expression and area of pericyte.Conclusion Results imply that pericyte regulate the angiogenesis through TGFβ 1 secretion.
作者 王医术 周洪澜 李玉林 李一雷 王心蕊 张丽红 吴珊
机构地区 吉林大学基础医学院病理教研室 吉林大学中日联谊医院
出处 《中国实验诊断学》 2004年第2期128-130,共3页 Chinese Journal of Laboratory Diagnosis
基金 国家自然科学基金资助项目 ( 3 9870 3 14 )
关键词 TGFΒ1 周细胞 乳腺癌 血管生成 手术切除 肿瘤 breast cancer pericyte TGFβ 1 angiogenesis
分类号 R737.9 [医药卫生—肿瘤]
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参考文献10

  • 1王医术,mail.nbums.cc.jl.cn,李玉林,朱桂彬,王心蕊,吴珊,张丽红,高绪兰.乳腺癌间质新生血管周细胞的形态学特点及其意义[J].中华病理学杂志,2000,29(3):176-179. 被引量：14
  • 2Ato Y, Tsuboi R, Lyon R, et al. Characterization of the Activation of Latent TGF-β by Co-cultures of Endothelial Cells and Pericytes or Smooth Muscle Cells: A Self-regulating System[J] .The Journal of Cell Biology.1990,111:757.
  • 3Roberts AB, Sporn MB, Assoian RK, et al. Transforming growth factor β: Rapid induction of fibroblast and angiogenesis in vitro and stimulation of collagen formation in vitro[J]. Proc Natl Cad Sci (USA), 1986,83:4167.
  • 4Sanchez-Elsner T, Botella LM, Velasco B, et al. Synergistic cooperation between hypoxia and transforming growth factor-beta pathways on human vascular endothelial growth factor gene expression[J] .J Biol Chem,2001,276(42): 38527.
  • 5Fujimoto KS. Pericyte-Endothelial Gap Junctions in Developing Rat Cerebral Capillaries: A Fine Structural Study[J]. The Anatomical Record,1995,242:562.
  • 6Alicia O, D'Amore PA. Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells[J]. The Journal of Cell Biology. 1987,105:1455.
  • 7Teraoka H, Sawada T, Nishihara T, et al. Enhanced VEGF production and decreased immunogenicity induced by TGF-beta 1 promote liver metastasis of pancreatic cancer[J]. Br J Cancer, 2001,85(4) :612.
  • 8Breier G, Blum S, Peli J, et al. Transforming growth factor-beta and Ras regulate the VEGF/VEGF-receptor system during tumor angiogenesis[J].Int J Cancer,2002,97(2): 142.
  • 9Antonelli-Orlidge A, Kim BS, Sander RS, et al. An activated form of transforming growth factor β is produced by cocultures of endothelial cells and pericytes[J] .Proc Natl Acad,1989, 86:4544.
  • 10Vang EY, Moses HL. Transforming growth factor β1-induced changes in cell nigration proliferation and angiogenesis in chick chorioallantoic membrane[J].J Cell Biol, 1990,111:731.

二级参考文献8

  • 1Nicosia RF,Villaschi S.Rat aortic smooth muscle cells become pericytes during angiogenesis in vitro[].Laboratory Investigation.1995
  • 2Fujimoto K.Pericyte-endothelial gap junctions in developing rat cerebral capillaries: a fine structural study[].The Anatomical Record.1995
  • 3Verbeek MM,Otte-Holler I,Wesseling P,et al.Induction of alphasmooth muscle actin expression in cultured human brain pericytes by transforming growth factor-beta 1[].American Journal of Pathology.1994
  • 4SatoN,SawasakiY,SenooA,etal.Developmentofcapillarynetworksfromratmicrovascularfragmentsinvitro :theroleofmyofibroblasticcells[].Microvascular Research.1987
  • 5Nehls V,Drenckhahn D.The versatility of microvascular pericytes: from mesenchyme to smooth muscle[].Histochemistry.1993
  • 6Wong HC,Boulton M,Marshall J,et al.Growth of retinal capillary endothelia using pericyte conditioned medium[].Investigative Ophthalmology.1987
  • 7OrlidgeA,D′AmorePA.Inhibitionofcapillaryendothelialcellgrowthbypericytesandsmoothmusclecells[].JCellBiol.1987
  • 8Sato Y,Rifkin DB.Inhibition of endothelial cell movement by pericytes and smooth muscle cells: activation of a latent transforming growth factor-β1-like molecule by plasmin during co-culture[].The Journal of Cell Biology.1989

共引文献13

同被引文献26

  • 1Hirschi KK, D'Amore PA. Pericytes in the microvasculature. Cardiovasc Res,1996,32(4):687～698.
  • 2Nehls V, Drenckhahn D. Heterogeneity of microvascular pericytes for smooth muscletype alpha-actin. J Cell Biol,1991,113(1):147～154.
  • 3Sims DE. Diversity Within Pericytes.Clin Exp Pharmacol Physiol,2000,27 (10):842～846.
  • 4Sims DE. The pericyte.Tissue and Cell,1986,18(2):153～174.
  • 5Tilton RG, Kilo C, Williamson JR.Microvasc Res,1979,18(3):325～335.
  • 6Kelley C, D'Amore P, Hechtman HB,et al.Microvascular pericyte contractility in vitro: comparison with other cells of the vascular wall. J Cell Biol,1987,104(3):483～490.
  • 7Allt G,Lawrenson G.Periyte:Cell bioloy and pathology.Cell Tissue Organs,2002,169(1):1～11.
  • 8Martin AR, Bailie JR, Robson T,et al.Retinal pericytes control expression of nitric oxide synthase and endothelin-1 in microvascular endothelial cells.Microvasc Res,2000,59(1):131～139.
  • 9Hellstrm M, Kalén M, Lindahl P,et al. Role of PDGF-B and PDGFR-B in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse. Development,1999,126(14):3 047～3 055.
  • 10Hudes GR,Li WY,Rockey JH,et al. Prostacyclin is the major prostaglandin synthesized by bovine retinal capillary pericytes in culture. Invest Ophthalmol Vis Sci,1988,29(10):1 511～1 516.

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中国实验诊断学
2004年 第2期

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