摘要
目的 构建及包装由全长 5 1kbAFP启动子和低氧反应元件 (HRE)与 0 3kbAFP启动子两者组合的杂合0 3kbAFP启动子控制下表达HIVvpr基因的两种重组非复制型腺病毒。方法 采用细菌内同源重组腺病毒载体制备方法和PacI酶切、PCR、SouthernBlot鉴定方法。结果 构建及包装出由这两种AFP启动子控制下表达HIVvpr基因的重组非复制型腺病毒 ,经PacI酶切、PCR和SouthernBlot基因整合分析证明构建成功。结论 我们成功构建了的全长5 1kbAFP启动子和HRE与 0 3kbAFP启动子两者组合的杂合 0 3kbAFP启动子控制下表达HIVvpr基因的重组非复制型腺病毒 。
Objective To construct the recombinant replication-incompetent adenovirous of expressing HIV vpr gene drived by the 5 1kb AFP promoter and the 0 3kb AFP promoter linked with HRE. Methods Two recombinant replication-incompetent adenovirous were identified by the PCR and Southern Blot and approved its right. Results Two recombinant replication-incompetent adenovirous were constructed successfully. Conclusion Our work pave the way for application of two recombinant replication-incompetent adenovirous for the special-gene therapy of the hepatocellular carcinoma.
出处
《中国实验诊断学》
2004年第2期132-134,共3页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金项目 编号 :3 0 0 0 761748