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两种不同AFP启动子控制下表达HIV vpr基因的重组非复制型腺病毒获得及鉴定 被引量：2

Construction and identify the recombinant replication-incompetent adenovirous of expressing HIV vpr gene drived by the two AFP promoter
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摘要 目的　构建及包装由全长 5 1kbAFP启动子和低氧反应元件 (HRE)与 0 3kbAFP启动子两者组合的杂合0 3kbAFP启动子控制下表达HIVvpr基因的两种重组非复制型腺病毒。方法　采用细菌内同源重组腺病毒载体制备方法和PacI酶切、PCR、SouthernBlot鉴定方法。结果　构建及包装出由这两种AFP启动子控制下表达HIVvpr基因的重组非复制型腺病毒 ,经PacI酶切、PCR和SouthernBlot基因整合分析证明构建成功。结论　我们成功构建了的全长5 1kbAFP启动子和HRE与 0 3kbAFP启动子两者组合的杂合 0 3kbAFP启动子控制下表达HIVvpr基因的重组非复制型腺病毒 。 Objective To construct the recombinant replication-incompetent adenovirous of expressing HIV vpr gene drived by the 5 1kb AFP promoter and the 0 3kb AFP promoter linked with HRE. Methods Two recombinant replication-incompetent adenovirous were identified by the PCR and Southern Blot and approved its right. Results Two recombinant replication-incompetent adenovirous were constructed successfully. Conclusion Our work pave the way for application of two recombinant replication-incompetent adenovirous for the special-gene therapy of the hepatocellular carcinoma.
作者 魏强 李凡 王健伟 郭丽 洪涛
机构地区 吉林大学基础医学院 中国疾病预防控制中心病毒病预防控制所
出处 《中国实验诊断学》 2004年第2期132-134,共3页 Chinese Journal of Laboratory Diagnosis
基金 国家自然科学基金项目 编号 :3 0 0 0 761748
关键词 AFP启动子 HIV VPR基因 基因表达 非复制型腺病毒 低氧反应元件 HRE 治疗 AFP promoter HIV vpr recombinant adenovirus
分类号 R346 [医药卫生—基础医学]
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参考文献9

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共引文献5

同被引文献32

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引证文献2

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中国实验诊断学
2004年 第2期

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