短暂脑缺血发作患者血小板膜糖蛋白CD61、CD62P表达及阿斯匹林干预的临床研究

Clinical trial on the expression of platelet membrane glycoproteins CD61,CD62P and the effect of aspirin among patiens with TIA

目的 观察短暂脑缺血发作 (TIA)患者外周血血小板膜糖蛋白CD6 1、CD6 2P的表达及其临床意义 ,并探讨阿司匹林对血小板表面膜糖蛋白的作用。方法 应用流式细胞术 ,用单克隆抗体作分子探针测定 2 0例TIA患者的CD6 1、CD6 2P的分子表达 ,包括进展及未进展为脑梗塞的病人 ,并依其是否常规口服阿司匹林将其分两组 ,患者组内及患者与 18例健康成年人所测结果进行比较。结果 (1)TIA组血小板CD6 2P阳性表达率与健康对照组比较P <0 0 1;(2 )进展为脑血栓形成的患者与未进展为脑血栓形成的患者比较P <0 0 1;(3)TIA组常规口服阿司匹林患者与未常规口服阿司匹林的患者相比P <0 0 1;(4)TIA组男性与女性血小板CD6 2P阳性表达率相比P >0 0 5。结论 血小板活化是短暂脑缺血发生的重要因素 ,且活化程度越高 ,越容易形成血栓 ,而CD6 1、CD6 2P能较好反映血小板活化 ,故可较早预测TIA并估计其演变趋势 ;同时 ,阿司匹林对CD6 1、CD6

Objective To observe expression changes of platelet membrane glycoproteins(MG) CD61、CD62P in peripheral blood of patients with transient ischemic attack(TIA) and its clinical significance, and to research the effects of aspirin on platelet MG. Methods To mensurate CD61、CD62P expression of 20 cases of TIA patients with flowcytometry(FCM) and monoclonal antibody, including group progressing to infarction and non-progressing group. Whether taking aspirin orally or not, samples were divided into 2 groups. And compare the results between groups and compare with those of 18 cases of healthy volunteers. Results Result The expression of CD61、CD62P in healthy volunteers was lower while that of TIA patients was higher P <0 01; To compare positive percentage of patients progressing to infarction , and that of nonprogressing patients P <0 01;After TIA patients took orally aspirin more than 6 months regularly, the activation rate of platelet and that of patients without aspirin taken orally, P <0 01; To compare that of male TIA patients and female, P >0 05.Conclusion Activation of platelet is an important factor to the onset of TIA, and the higher activation is, the easier thrombosis is formed. CD61、CD62P is able to reflect the activation of platelet so as to forecast the onset of TIA and to estimate its evolvement trend; meanwhile, aspirin plays a remarkable suppressive effect on CD61、CD62P expression.