离体培养条件下神经生长因子对毛囊的影响

Effects of nerve growth factor on human hair follicles in vitro

目的 观察神经生长因子 (nervegrowthfactor,NGF)对离体培养条件下的人头皮毛囊生长的影响。方法 在离体人头皮毛囊培养模型中 ,加入 10 0 μg·L -1的NGF ,测量毛囊长度的变化以及 2 4hDNA合成率。结果 目镜测微器观测 10 0 μg·L -1的NGF与 12 5mg·L-1的米诺地尔一样能明显地促进游离的人头皮毛囊生长 (P <0 .0 5 ) ,尤以前 8d较为明显 ;3 H TdR掺入检测的DNA合成率亦明显增高。结论 10 0 μg·L

Objective To observe the effects of nerve growth factor (NGF) on growth of human hair follicles in vitro. Methods NGF was added to the model of human hair follicles in vitro. The length and the DNA synthesis rate of human hair follicles in vitro were measured. Results We found both 100 μg·L -1 NGF and 125 mg·L -1 minoxidil significantly accelerated the growth of human hair follicles in vitro (P< 0.05), especially in the previous 8 days. The rate of incorporation of 3H-TdR was obviously higher than that of the control. Conclusion NGF of 100 μg·L -1 significantly promotes the growth of human hair follicles in vitro.