子宫内膜腺癌中p53和CDK4的表达及意义

The expression and significance of p53 and CDK4 in human endometrial adenocarcinoma

目的 探讨子宫内膜腺癌中调亡相关基因p5 3和细胞周期素依赖性激酶 4 (cyclindependentkinase 4 ,CDK4 )的表达水平及临床意义。方法 采用免疫组化SP法检测 32例子宫内膜腺癌中p5 3和CDK4的表达。结果 32例子宫内膜腺癌中p5 3蛋白表达阳性率为 4 3.8% ,p5 3阳性表达与子宫内膜腺癌的组织学分级 ,肌层浸润深度和淋巴结转移呈负相关 (P <0 .0 5 ) ,与临床分期无相关关系 (P >0 .0 5 ) ;CDK4在子宫内膜腺癌中的阳性表达率为 6 8.8% (2 2 /32 ) ,CDK4阳性表达与子宫内膜腺癌的浸润深度和淋巴结转移呈正相关 (P >0 .0 5 ) ,与组织学分级和临床分期均无关(P >0 .0 5 ) ,p5 3和CDK4的表达呈负相关 (P <0 .0 5 )。结论 p5

Objective To investigate the expression and significance of p53 and CDK4 proteins in human endometrial adenocarcinoma. Methods Expressions of p53 and CDK4 proteins were detected by immunohistochemical technique in 32 cases of endometrial adenocarcinoma. Results The positive expression rate of p53 protein in the 32 cases of endometrial adenocarcinoma was 43.8% (14/32), and the positive rate of p53 in endometrial adenocarcinoma was negatively related to pathological grade, myometrial invasion and lymph node metastasis (P< 0.05), but unrelated to clinical stage (P> 0.05). The positive expression rate of CDK4 was 68.8% (22/32), and CDK4 expression was significantly associated with myometrial invasion and lymph node metastasis (P< 0.05), but unrelated to pathological grade or clinical stage (P> 0.05) . There was negative correlation between p53 and CDK4 (P< 0.05). Conclusion p53 and CDK4 as cell cycle regulatory factors may be involved in carcinogenesis and development of endometrial adenocarcinoma.