中国汉族、维吾尔族、哈萨克族右美沙芬氧化代谢多态性表型研究

Study on the phenotype in the metabolic polymorphism of dextromethorphan in Han, Urghur, Hasake nationalities

目的 以右美沙芬为探针药,对中国汉族、维吾尔族、哈萨克族人群药物氧化代谢的多态性进行研究。方法 3 84名汉族、维吾尔族、哈萨克族健康受试者口服右美沙芬片30 mg后,取8 h内全部尿样,尿样经酶水解及酸、碱提取纯化后,荧光检测右美沙芬及去甲右美沙芬浓度,以其比值(MR)对3个民族人群右美沙芬氧化代谢多态性的表型分型。结果 右美沙芬氧化代谢慢代谢者频发率:汉族为0.76％,维吾尔族为8.09％,哈萨克族为1.72％。结论 汉族与维吾尔族比较,慢代谢者频发率有显著性差异(P<0.0 1);哈萨克族与维吾尔族比较,慢代谢者频发率有显著性差异(P<0.05);汉族与哈萨克族比较,慢代谢者频发率的无显著性差异。

Objective To study the metabolic polymotphism of dextromethorphan in Chinese population of Han, Urghur, Hasake nationalities. Methods Three hundred eighty four healthy volunteers were into 3 groups (Han, Urghur and Hasake groups). All volunteer' s urine samples within 8 h after a single oral dextromethorphan dose of 30 mg were collected. The concentration of dextromethorphan and dextrophan were measured by HPLC with fluorescence dedector. The metabolic ratio (MR) of the two metabolites was used to the classification of metabolic polymorphism of dextromethorphan in the three nationalities. Results The incidences of poor metabolizers of dextromethorphan in Han, Urghur and Hasake nationalities is 0.76%,8.09%, 1.72%, respectively. Conclusion The incidence of poor metabolizers between Han and Urghur populations shown significant statistical differences (P <0.01), and the incidence of poor metabolizers between Hasake and Urghur populations also shown statistical differences (P<0.05), but no statistical difference in the incidence of poor metabolizers between Han and Hasake populations(P>0.05).