脑梗死患者凝血因子Ⅴ基因3种多态性和APC-R、LA的检测

The Analysis Three-Point of Coagulation Factor Ⅴ Gene Polymorphism,APC-R and LA in Cerebral Infarct Patients

目的 :研究脑梗死患者狼疮抗凝物质 (LA)、活化蛋白C抵抗 (APC R)和凝血因子 Ⅴ 基因 3种多态性的关系。方法 :测定脑梗死患者和正常对照血浆中LA、APC R ,并用限制性内切酶片段多态性方法测定FⅤ Gl691→A、G10 91→C、A10 90→G等 3种基因多态性的发生情况。结果 :脑梗死患者LA阳性占 45 5 6% ,APC R阳性占 3 3 3 % ,但 3种基因多态性均未见阳性。结论 :脑梗死患者LA阳性率明显高于正常对照。LA可能是引起脑梗死患者脑血栓形成的重要原因之一。脑梗死患者存在APC R ,但可能和凝血因子 Ⅴ 基因 3种多态性无关。

Objective: To investigate the relationship of gene polymorphism of coagulation factor Ⅴ with activated protein C-resistance(APC-R )and lupus anticoagulant(LA) in cerebral Infarct patients. Methods: APC-R ,LA and FⅤ G1691→A,G1091→C,A1090→ G gene polymorphism were assayed in 90 cer ebral Infarct patients and 60 normal subjects. Results: Positive LA patients w ere 41,there were 3 patients with APC-R and no FⅤ G1691 →A,G1091→C,A1090→G gene mutation in cerebral Infarct patients. Conclusion: There were especially high positive LA rate and perhaps LA is one of the import ant causes of thrombos is in patient with cerebral infarct. There are APC-R phenomenon in cerebral inf arct patients,but perhaps having no relation with factor Ⅴ gene mutation.