胶质瘤中PKCα表达及与细胞增殖和凋亡的关系

Relation of PKCα,cellular proliferation and apoptosis in gliomas

目的 探讨人脑胶质瘤中PKCα的表达情况 ,以及与细胞增殖和凋亡的关系。方法 37例人脑胶质瘤标本 ,星形细胞瘤 2 5例和胶质母细胞瘤 12例 ,正常脑组织 10例作为对照。PKCα和Ki 6 7的检测用免疫组化 ,细胞凋亡用TUNEL。用Ki 6 7LI和AI对增殖和凋亡进行评估。结果 PKCα在胶质瘤及正常组织中均有表达 ,前者为 6 7.6 % ,后者为 30 .0 % ,二者有显著性差异 ,星形细胞瘤阳性率为 6 0 % ,胶质母细胞瘤为 83.3% ,不同肿瘤分型中PKCα表达有差异 ,PKCα表达与细胞增殖和生长显著相关 ,与凋亡无明显相关。结论 人脑胶质瘤中PKCα过度表达 。

Objective To study the alteration of protein kinase Cα(PKCα) in gliomas, analyze the relationship with cellular proliferation and apoptosis.Methods Thirty seven gliomas were classed by WHO standard (1990) as astrocytomas (25 cases, including fibrillary, 7 cases; protoplasmic, 6 cases; anaplastic, 12 cases), glioblastomas (12 cases, including GBM, 4 cases). Ten normal brain tissues were taken as control. The expression of PKCα and cell proliferation marker, Ki 67 were detected by immunohistochemical method. Cellar apoptosis was detected by TUNEL. Cellar proliferation and apoptosis were assessed by Ki 67 label index (Ki 67 LI) and apoptotic indices (AI). Results PKCα was expressed in gliomas as well as normal brain tissues. The former was 67.6% (25/37), while the later was 30.0% (3/10), there was significant difference between them ( P =0.04). The positive rate was 60% (15/25) in astrocytomas , and 83.3% (10/12) in glioblastomas, it was different among tumor types ( P =0.018). PKCα expression was associated with Ki 67 LI and cell overall daily growth (KP), but was not with apoptotic indices (AI).Conclusion PKCα was overexpressed in gliomas, and related with tumorigenesis and pathogenesis closely, which was basic theory to treating gliomas with PKC inhibitors.