MMP-2及MMP-9表达在侵袭性垂体腺瘤中的生物学意义

The biology behavior of matrix metalloproteinase-2 and 9 expression in invasive cavernous pituitary adenomas

目的 本研究的目的是探讨基质金属蛋白酶 2 (matrixmetalloproteinase 2 ,MMP 2 )及基质金属蛋白酶 9(matrixmetalloproteinase 9,MMP 9)的表达与侵袭性垂体腺瘤相关性研究。以寻找垂体腺瘤侵袭性可能的发病机理。方法 用免疫组化方法对 5 4例垂体瘤患者的组织标本进行研究。并对其中 16例患者采用逆转录 聚合酶链反应 (RT PCR)的方法对MMP 2及MMP 9mRNA的表达进行分析。结果 5 4例垂体瘤患者中 ,有 32例女性 ,2 2例男性。平均年龄为 4 9.9岁 (从 18～ 76岁 )。其中 ,12例为侵袭性垂体腺瘤 ,4 2例为非侵袭性垂体腺瘤。免疫组化显示 ,侵袭性垂体腺瘤的MMP 2及MMP 9的表达明显高于非侵袭性垂体腺瘤 (3.9± 0 .5 ;2 .3± 0 .2 ;P <0 .0 1)和 (4.1± 0 .4 ;2 .6± 0 .2 ;P <0 .0 1)。MMP 2及MMP 9的表达与MIB 1的表达无明显相关性 (r=$C 0 .0 5 ;P >0 .0 5 )。侵袭性垂体腺瘤MMP 2及MMP 9mRNA的表达 ,明显高于非侵袭性垂体腺瘤 [(5 9.7± 12 .5 ) % ;(33.3± 5 .4 ) % ,P <0 .0 5 ]及 [(6 8.2± 15 .3) % ;(2 1.8± 8.2 ) % ;P <0 .0 5 ]。结论 MMP 2及MMP 9的高表达与肿瘤的侵袭性密切相关。但与肿瘤的大小及分泌功能无明显关系。MMP 2及MMP 9可以作为肿瘤侵袭性一项有效的指标。

Objective The aim of this study is to evaluate the relationship between the expression of matrix metalloproteinase(MMP) 2 and 9 and the cavernous invasion of pituitary adenomas.Methods The expression of MMP protein of tissue samples from 54 pituitary adenomas were examined by immunohistochemical method. In sixteen cases, the expression of MMP 2 and MMP 9 mRNA were also examined by RT PCR assay.Results Thirty four patients were women and 20 were men, with a mean age of 49.9 years old (range 18～76 years). There were 12 cases with cavernous sinus invasion, and 42 were noninvasive cases. MMP 2 and MMP 9 score of invasive case (3.9±0.5, 4.1±0.4) were significantly higher than those (2.3±0.2; P <0.01; 2.6±0.2; P < 0.01 ) without invasion. The MIB 1 LI of this study presented no significant difference between the invasive and noninvasive pituitary adenomas. The percentage of MMP 2 mRNA/β actin mRNA and MMP–9 mRNA/β actin mRNA were also observed significantly higher in invasive pituitary adenomas [(68.2±15.3)%; (59.7±12.5 )%] than noninvasive pituitary adenomas [(21.8±8.2)%, P <0.05; (33.3±5.4)%, P <0.05]. Conclusion Our study suggests that the expression of MMP 2 and MMP 9 may have a value to assess the invasive pituitary adenomas , and proliferation and invasion of pituitary adenomas may present a different mechanism.