摘要
目的 观察腺病毒介导的NT 3基因在培养雪旺细胞 (Schwanncells,SCs)的表达。方法在2 93细胞中培养扩增NT 3重组腺病毒 (adenovirusvectorforNT 3,Ad NT 3) ,用组织培养半数感染量法测定其滴度。然后用Ad NT 3感染原代培养的SCs,逆转录酶 多聚酶链反应 (RT PCR)技术检测NT 3基因的表达。结果 Ad NT 3扩增后获得了较高滴度的病毒。SCs经NT 3重组腺病毒感染 2 4h后有NT 3mRNA的转录。结论 腺病毒介导的NT
Objective To investigate expression of neurotrophin 3(NT 3)gene in cultured Schwann cells introduced by adenoviral vector in vitro.Methods The recombinant adenoviral vector for NT 3(Ad NT 3)was propagated in 293 packaging cells. Viral titers were determined by tissue culture infectious dose 50 (TCID 50 ) methods . The primary culture and purification of Schwann cells was established and the efficiency of transfection of Ad NT 3 was determined by reverse transcriptase polymerase chain reaction(RT PCR).Results High titers of recombinant adenoviral particles after propagation of Ad NT 3 in 293 cells were obtained. NT 3 mRNA transcription could be detected by RT PCR in SCs 24 hours following infection with Ad NT 3.Conclusion It is demonstrated that Ad NT 3 transfected SCs can express NT 3 mRNA.
出处
《中华神经外科疾病研究杂志》
CAS
2004年第1期61-63,共3页
Chinese Journal of Neurosurgical Disease Research
基金
国家自然科学基金资助项目 (30 2 71 31 5)
