白细胞介素6 mRNA在突出腰椎间盘中的表达及临床意义

Study on the expression of IL-6 mRNA and clinical significance in the herniated disc of the lumbar spine

目的 探讨白细胞介素 6 (IL 6 )mRNA在突出腰椎间盘组织的表达及临床意义。方法 以RT PCR技术测定不同类型椎间盘髓核、纤维环、软骨终板组织标本IL 6mRNA表达。结果椎间盘突出症组IL 6mRNA表达水平明显高于正常对照组 ,差异非常显著 (P <0 0 0 1)。病变组髓核、纤维环、软骨终板不同部位IL 6mRNA的表达有显著差异 (P <0 0 5 )。根据NorbetBoos的病理组织学分级 ,轻度病变者 11例 ,重度者 14例 ,重度组IL 6mRNA的表达明显高于轻度者 ,有显著统计学意义。不同突出病理类型椎间盘突出类椎间盘型IL 6mRNA的表达有显著差异 ,游离型高于突出型 ,突出型高于凸起型 ,有显著统计学意义。髓核组织中IL 6mRNA的表达与患者的直腿抬高程度 (SLR)呈负相关 (γ =- 0 4 5 7,P =0 0 0 3)。在不同病程阶段患者椎间盘组织中IL 6mRNA的表达无明显差异。结论 IL 6参与腰椎间盘突出的病理过程 ,随病情的加重而分泌增多。IL

Objective To investigate the expression of IL 6 mRNA in herniated disc specimens and to study the clinical.Significance on the expression of IL 6mRNA of lumbar disc herniation. Methods Lumbar discs were subdivided into three kinds of samples:nucleus pulposus,annulus fibrosus and cartilaginous endplates.Expression of IL 6 mRNA was investigated in discs specimens by using reverse transcriptase polymerase chain reaction(RT PCR).Results IL 6 mRNA was overexpressed in herniated disc group compared with those in controlled group( P< 0 001).The expression of IL 6 mRNA was different in three parts of the herniated lumbar disc( P< 0 05),and nucleus pulposus had the highest level among the three parts.Three different types of herniation have different expressions of IL 6 mRNA statistically ( P< 0 05).But the expression of IL 6 mRNA showed no marked difference in different phases of the disease ( P> 0 05).According to Boos's Classification System for Grading Histologic Changes in the Intervertebral Disc,patients were divided into two groups,in which 14 were serious cases and the other 11 mild.The amplification result showed a higher expression of IL 6 mRNA in serious degenerated disc than in mild degenerated disc( P< 0 001).A correlation was found between the expressions of IL 6 mRNA in herniated nucleus pulposus and the degree of the straight leg raising test ( r= -0 457, P= 0 003).Conclusion IL 6 plays a critical role in the development of lumbar disc degeneration and herniation.The expressions of IL 6 mRNA in herniated disc specimens are correlated with pathogenesis of radicular pain.