聚合酶链反应快速检测丝状真菌

Rapid Detection of Molds by PCR Assay

目的 建立并评价一种新的丝状真菌的快速PCR检测方法。方法 将 13种培养的不同丝状真菌菌种经匀浆器研磨处理后直接放入一个新建立的PCR体系 ,用 2对真菌通用引物进行PCR扩增。结果 13种不同的丝状真菌菌种均在 2h内通过该PCR方法成功地获得了靶DNA的扩增 ,无需任何DNA抽提步骤 ,可用于直接扩增处于任何生长期的未经处理的丝状真菌DNA。结论 此法简便、快速 ,敏感性、特异性均高 ,对丝状真菌引起的深部真菌感染的早期诊断将具潜在意义。

Objective To set up and evaluate a method for rapid detection of molds by PCR assay.Methods 13 species of molds cultured were directly disposed with a new established PCR system,which includes two pairs of universal fungal primers,and then PCR was carried out.Results The target DNA from all 13 different molds studied were successfully amplified by PCR based approach within 2 hours,not needing any steps of DNA extraction.The described PCR system could be directly amplified untreated molds on every living stage.Conclusion This method is rather simple,rapid and of high sensitivity and specificity,which has potential value in the early diagnosis of the molds inducing deep fungal infections.