兔血管平滑肌细胞在低剂量电离辐射后的分子生物学变化

Molecular biology changes of vascular smooth muscle cells in the cultured rabbits after a low dose of ionizing radiation

目的:观察体外培养的兔动脉平滑肌细胞(smoothmusclecells,SMCs)对γ射线照射的分子生物学机制。方法:取新西兰白兔(n=2)SMCs培养至第5～7代,对静止期SMCs分别给予γ射线2.5,5.0,10.0,20.0及30.0Gy一次性照射后继续培养4,24或72h。通过原位杂交方法观察γ射线对SMCs的c-fos与p53基因表达的影响。结果:低剂量γ射线照射后早期(4h左右)SMCsc-fosmRNA表达减少,p53mRNA表达增加,而高剂量γ射线照射及低剂量γ射线照射24h及72h时c-fos与p53基因表达无明显变化。结论:SMCs在低剂量电离辐射后,早期发生了c-fos与p53基因改变,c-fos与p53基因的改变可能参与了γ射线抑制SMCs的增殖过程。

AIM:To investigate the molecular biological mechanisms of artery smooth muscle cells (SMCs) in rabbits cultured in vitro by γ radiation. METHODS:New Zealand rabbits'(n=2) artery SMCs were cultured for passage 5-7 and one time irradiation on SMCs with various doses of 2.5,5.0,10.0,20.0 and 30.0 Gy was taken in quiescent period for the following cultivation at 4,24 and 72 h respectively. Situ mRNA hybridization was used to observe the effect of γ radiation on the c fos and p53 gene expression of SMCs. RESULTS:After a low dose (2.5-5.0 Gy) of γ radiation, the mRNA expression of c fos decreased in early time(about 4 hours), while that of p53 increased.There was no significant difference between c fos and p53 gene expressions of a high dose of radiation at anytime and a low dose of radiation at 24 or 72 h.