摘要
目的 检测Hoxd 13基因在肛门直肠畸形病人和正常人的直肠末端表达状况 ,探讨Hoxd 13基因与人类先天性肛门直肠畸形发生的关系。方法 应用RT -PCR方法检测Hoxd 13基因在 16例肛门直肠畸形患儿和 5例正常儿童直肠末端的表达水平。结果 Hoxd 13基因mRNA在先天性无肛畸形直肠末端表达水平明显低于正常直肠末端的表达 (0 .32± 0 .36vs 0 .73± 0 .10 )。 (P <0 .0 1)。结论 Hoxd
Objective To study the Hoxd 13 gene expression in the children with anorectal malformation (CAM) and normal children, and to explore the relationship between Hoxd 13 gene and CAM. Methods Hoxd 13 gene mRNA expression was detected by RT-PCR in the rectal pouch in both 16 cases of anorectal malformations and 5 normal children. Results The expression level of Hoxd 13 gene mRNA was significantly higher in normal children than that in children with CAM ( 0.32 ± 0.26 vs 0.73 ± 0.10 ), (P< 0.01 ). Conclusions Hoxd 13 gene expression may be involved in the pathogenesis of anorectal malformation.
出处
《中国当代儿科杂志》
CAS
CSCD
2003年第3期201-204,共4页
Chinese Journal of Contemporary Pediatrics