P物质与哮喘的关系(英文)

Relationship Between Substance P and Asthma

目的 动态研究哮喘患儿血浆及哮喘豚鼠血液、支气管肺泡灌洗液 (BALF)和肺组织中P物质(SP)含量及SPmRNA表达变化 ,探讨SP与小儿哮喘发病机制的关系。方法 用放射免疫方法 ,动态测定 35例不同严重程度哮喘患儿急性发作期及其临床症状缓解期的血浆SP含量变化 ;并在豚鼠哮喘模型上同期进行了不同严重程度哮喘豚鼠血液、BALF和肺组织中SP含量的对比研究 ,利用RT -PCR方法研究肺组织SPmRNA表达变化。结果 ①哮喘患儿急性发作期血浆SP含量较其自身症状缓解期及正常对照组均明显增高 ,差异有显著性(P <0 .0 1) ;症状航馄谘 P含量较正常对照组差异无显著性 (P >0 .0 5 ) ;②重症哮喘急性发作期血浆SP含量 ,较轻、中度哮喘急性发作期的含量显著增高 ,差异有显著性 (P <0 .0 5 ) ;③哮喘豚鼠血液、BALF和肺组织中SP含量较正常豚鼠均显著增高 (P <0 .0 1) ,反复诱喘豚鼠各组织中SP含量均显著高于诱喘一次豚鼠 (P <0 .0 5 ) ;④哮喘豚鼠肺组织内SPmRNA表达较正常组显著增高 ,反复诱喘豚鼠SPmRNA表达显著高于诱喘一次的豚鼠 (P <0 .0 1)。结论 哮喘患儿及哮喘豚鼠SP含量增加 ,哮喘豚鼠肺组织中SPmRNA的表达明显上调 ,均与病情呈显著正相关。SP与小儿哮喘关系密切 。

Objective To study the changes of substance P (SP) content in plasma of asthmatic children and in the blood, bronchoalveolar lavage fluid (BALF) and lung tissues of asthmatic guinea pigs and SP mRNA expression, and to investigate the relationship between SP and asthma of children. Methods We determined the contents of plasma SP by the method of immunoassay at the stage of acute attack and clinical remission in 35 children with asthma. On the basis of asthma model of guinea pigs, we examined the contents of SP in the blood, BALF and lungs. The expression of SP mRNA in lungs was examined by RT PCR. Results ① At the stage of acute attack, the level of SP was higher than that of the stage of clinical remission and normal controls (P< 0.01 ). At the stage of clinical remission, the difference of SP contents was not obvious compared with that of controls (P> 0.05 ). ② The SP content of severe asthmatic children was higher than that of mild asthmatic children (P< 0.05 ). ③ In asthmatic guinea pig groups, SP contents in the blood, BALF and lungs were higher than those of normal ones (P< 0.01 ), and the expressions of SP mRNA in lungs were higher (P< 0.01 ). In repeatedly attack group, the SP content of guinea pigs was higher than that of asthma group (P< 0.05 ), and the expression of SP mRNA in lungs was also higher (P< 0.01 ). Conclusions SP levels increased in asthmatic children and asthmatic guinea pigs, and the expression of SP mRNA was up regulated in the lungs of asthmatic guinea pigs. They were positive related to the severity of asthma. So SP has close relationship with asthma and may play a role in the mechanism of asthma.