同型半胱氨酸对大鼠胚胎发育的毒性作用

TOXIC EFFECT OF HOMOCYSTEINE ON THE RAT EMBRYONIC DEVELOPMENT

目的 研究同型半胱氨酸 (homocysteine ,HCY)和同型半胱氨酸诱导基因 (homocysteine inducedgene ,HCY 2 )与心脏畸形的关系。 方法 大鼠妊娠 8、9、10 3d尾静脉注射同型半胱氨酸 ,分别于第 13、15、17、19d取材 ,用组织学和免疫组织化学ABC法观察心脏畸形和基因Hcy 2在心脏上的表达并进行图像定量分析。 结果HCY对大鼠胚胎心脏有明显致畸作用 ,主要为心脏偏位 ,心脏过小 ;室间隔缺损 ,心房缺失等 ;ABC法显示基因Hcy 2在给药组胚胎心脏中表达增强。 结论 HCY对心脏发育是一种新的毒性因子 ;Hcy 2基因的过度表达与心脏畸形发生可能有一定关系。

Objective To study the relationship between homocysteine (HCY),homocysteine-induced gene(HCY-2) and heart defects. Methods Pregnant rats were injected with D L-homocysteine 0.5*!μmol/L,i.v,once a day from day 8 to day 9 of pregnancy and they were sacrificed on day 13,15,17,19 separately;then the histological and immunohistochemical staining method and the technique of pictures assay were used to analyse the defects and the Hcy-2 expression in the embryonic hearts. Results The HCY could result in heart defects included ectopia cordis,hypoplastic heart,ventricular septal defect and the complete atrial absense;the expression of Hcy-2 was stronger in experimental groups than that in control groups.Conclusion Homocysteine may be a new heart-developing toxic factor,and there were relations between the excessive expression of Hcy-2 and the heart defects.