神经再生素对PC12细胞凋亡的影响

EFFECT OF NERVE REGENERATION FACTOR ONAPOPTOSIS OF PC12 CELLS

目的 研究神经再生素 (NRF)对PC12细胞凋亡的影响并初步探讨其机制。 方法 通过观察细胞形态的变化、MTT微量比色法、流式细胞仪AnnexinV和caspase 3活力检测等方法 ,了解NRF对低浓度血清培养下PC12细胞凋亡的影响。NGF和RPMI16 40作对照。 结果 培养细胞的突起生长数量和长度、MTT微量比色的A值、AnnexinV PE 7AAD显示的凋亡细胞比例、caspase 3活力的检测等指标 ,在NRF组和NGF组间无明显差异 ;而与空白对照组间差异有显著性意义。 结论 神经再生素对低血清培养下PC12细胞的凋亡有抑制作用。

Objective To study the effect of nerve regeneration factor(NRF) on apoptosis of PC12 cells and its mechanism. Methods PC12 cells under the condition of low concentration serum(0^3% newborn bovine serum in RPMI 1640 medium) were treated with NRF or NGF(both are concentrations of 0^01*!mg/L,0^1*!mg/L,and 1*!mg/L).The changes in the number and outgrowth in cultured PC12 cells were observed under light microscope,and the surival and growth of cultured PC12 cells were measured by MTT.The percentage of apoptosis or necrosis cells and caspase-3 activity were both detected by FCM. Results There were no difference in these parameters between the group of NRF and NGF,whereas there were superior to that of the blank control group.Conclusion NRF can protect the cultured PC12 cells from apoptosis in low concentration serum.