摘要
目的 研究GnRH对胃肠道内 5 HT分泌的影响。 方法 胃腔直接注射GnRH类似物阿拉瑞林(AlarelinGnRH A)以模拟外分泌产生的GnRH ,并以免疫组织化学、高压液相色谱电化学检测 (HPLC ECD)的方法对阿拉瑞林刺激后胃及十二指肠内 5 HT免疫反应细胞、血清中 5 HT含量进行检测。 结果 胃腔内注射GnRH A后 ,大鼠胃及十二指肠内 5 HT免疫反应阳性细胞密度显著增多 ,但血清中 5 HT含量显著减少。 结论 外分泌的GnRH对于 5 HT的释放起明显抑制作用 ,但对 5 HT的合成可能不产生影响。
Objective To study the effect of GnRH on the secretion of 5-HT in the stomach. Methods The GnRH-A Alarelin was directly injected into the stomach of rats to observe the change of density of 5-HT immunoreactive positive cell in the stomach and small intestine by immunohistochemical SABC method and 5-HT level in the circulating blood by HPLC-ECD, Results Compared with the control group,which was injected with saline into the stomach,the density of immunoreactive positive cell was significantly increased in the stomach and small intestine in the GnRH-A treatment group;while 5-HT level in the circulating blood was significantly reduced in the experimental group.Conclusion GnRH can inhibit the releasing of 5-HT but not change its synthesis.
出处
《解剖学报》
CAS
CSCD
北大核心
2003年第5期518-521,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 (39770 388)
