摘要
目的 探讨吗啡对钳夹损伤坐骨神经后其传入纤维终末在脊髓Ⅱ板层分布的影响。 方法 用显示抗氟化物酸性磷酸酶 (FRAP)组织化学方法 ,借助图像分析仪测量损伤坐骨神经后 15d和 30d吗啡组和对照组大鼠脊髓Ⅱ板层FRAP阳性反应物面积。 结果 损伤坐骨神经后吗啡组和对照组大鼠脊髓Ⅱ板层FRAP阳性反应区均有不同程度的缺失 ;对照组损伤坐骨神经后 30d的FRAP阳性反应物面积比 15d的FRAP阳性反应物面积增大 40 %。吗啡组损伤坐骨神经后 30d的FRAP阳性反应物面积比 15d的FRAP阳性反应物面积增大 2 2 %;同时也比对照组损伤坐骨神经后 30d的FRAP阳性反应物面积增大 19%。 结论 随着损伤坐骨神经后大鼠存活时间延长 ,其脊髓Ⅱ板层FRAP阳性反应物面积呈恢复性增大 ;吗啡能使坐骨神经损伤大鼠脊髓Ⅱ板层的FRAP阳性反应物面积明显增大。
Objective To explore the effects of morphine on the terminal of primary afferent fiber distributing in spinal lamina Ⅱ after the sciatic nerve crush. Methods The positive reactive areas of fluoride-resistant acid phosphatase(FRAP) at spinal lamina Ⅱ were measured by the FRAP histochemistry and microcomputer image analysis techniques, after sciatic nerve was injured 15 days and 30 days both in morphine and control groups of rats. Results The positive reactive areas of FRAP at spinal lamina Ⅱ were depleted on different degree in two groups of rat after sciatic nerve was injured. The positive reactive areas of FRAP were greater 40% on injured sciatic nerve in 30 days than in 15 days in control group. In morphine group, the positive reactive areas of FRAP were larger 22% on injured sciatic nerve in 30 days than in 15 days; simulaneously also were bigger 19% than on injured sciatic nerve 30 days of control group.Conclusion The positive reactive areas of FRAP at spinal lamina Ⅱ show recovering enlargement as the surviving time lengthens in both groups of rats injured sciatic nerve.Morphine may enhance the positive reactive area of FRAP at spinal lamina Ⅱ in rat of sciatic nerve crush.
出处
《解剖学报》
CAS
CSCD
北大核心
2003年第5期553-555,共3页
Acta Anatomica Sinica
基金
教育部留学回国人员科研启动基金 [教 (启 ) 99 2 81]
关键词
吗啡
大鼠
脊髓Ⅱ板层
受损伤坐骨神经
传入纤维终末
抗氟化物酸性磷酸酶
组织化学
Morphine
Injured sciatic never
Spinal lamina Ⅱ
Primary afferent fiber
Fluoride-resistant acid phosphatase (FRAP)
Histochemical study
Rat