抗Hu抗体在诊断系统性红斑狼疮中枢神经系统病变中的意义

Significance of anti-Hu antibodies in diagnosis of central nervous system diseases of systemic lupus erythematosus

目的 通过检测抗Hu抗体 (AHuA) ,评价其在诊断系统性红斑狼疮中枢神经系统病变 (CNS SLE)中的意义。方法 以神经马赛克生物薄片中神经元细胞为抗原 ,应用间接免疫荧光方法分别测定 182例病人和健康献血者血清 ,包括系统性红斑狼疮 (SLE)病人 96例 ,其中 2 0例为CNS SLE病人 ;其他结缔组织病病人 5 1例 ,其中 3例伴中枢神经系统病变 ;健康献血者 3 5例。结果 血清检测抗Hu抗体共 2 2例阳性 ,其中 17例为CNS SLE病人 ,敏感性 85 .0 % ( 17/2 0 ) ,特异性73 .9% ( 17/2 3 ) ;2例为系统性红斑狼疮非中枢神经系统病变 (非CNS SLE)病人 ,敏感性 2 .6% ( 2 /76) ;3例为其他结缔组织病病人 ,敏感性 5 .9% ( 3 /5 1) ,1例病人 1年后发展为SLE ,且出现精神症状。CNS SLE病人的抗Hu抗体阳性率明显高于非CNS SLE和其他结缔组织疾病者及健康献血者(P <0 .0 0 1)。结论 抗Hu抗体对CNS SLE的诊断有重要意义。

Objective To evaluate the diagnostic value of a nti-Hu antibodies (AhuA)in central nervous system diseases of systemic lupus er ythematosus(CNS-SLE).Methods 182 serum samples(96 with SLE in which 20 with CNS-SLE ,51 with other CTDs and 35 normal donors)were detected by using indirect immunof luorescence,on neurologic BIOCHIP MosaicTM.Results There were 22 serum samples shown positive tests,in whi ch 17 with CNS-SLE,with the sensitivity of 85.0%(17/20) and specificity of 73.9 %(17/23);2 positive test was non- CNS-SLE,with the sensitivity of 2.6%(2/76) ;3 positive tests were other CTDs,with the sensitivity of 5.9%(3/51),in which on e patient has become to SLE one year later, and show psychiatric disorders.The s ensitivity of CNS-SLE was significantly higher than that of non- CNS-SLE,oth er CTDs and normal donors ( P <0.001).Conclusion Detection of anti-Hu antibodies using neurologic B IOCHIP MosaicTM is a valuable test in the diagnosis of CNS-SLE.